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Frontline Science: COVID‐19 infection induces readily detectable morphologic and inflammation‐related phenotypic changes in peripheral blood monocytes
Journal of Leukocyte Biology ( IF 5.5 ) Pub Date : 2020-10-11 , DOI: 10.1002/jlb.4hi0720-470r
Dan Zhang 1 , Rui Guo 2 , Lei Lei 1 , Hongjuan Liu 3 , Yawen Wang 4 , Yili Wang 5 , Hongbo Qian 2 , Tongxin Dai 2 , Tianxiao Zhang 6 , Yanjun Lai 7 , Jingya Wang 8 , Zhiqiang Liu 8 , Tianyan Chen 9 , Aili He 10 , Michael O'Dwyer 11, 12 , Jinsong Hu 1, 12
Affiliation  

Excessive monocyte/macrophage activation with the development of a cytokine storm and subsequent acute lung injury, leading to acute respiratory distress syndrome (ARDS), is a feared consequence of infection with COVID‐19. The ability to recognize and potentially intervene early in those patients at greatest risk of developing this complication could be of great clinical utility. In this study, we performed flow cytometric analysis of peripheral blood samples from 34 COVID‐19 patients in early 2020 in an attempt to identify factors that could help predict the severity of disease and patient outcome. Although we did not detect significant differences in the number of monocytes between patients with COVID‐19 and normal healthy individuals, we did identify significant morphologic and functional differences, which are more pronounced in patients requiring prolonged hospitalization and intensive care unit (ICU) admission. Patients with COVID‐19 have larger than normal monocytes, easily identified on forward scatter (FSC), side scatter analysis by routine flow cytometry, with the presence of a distinct population of monocytes with high FSC (FSC‐high). On more detailed analysis, these CD14+CD16+, FSC‐high monocytes show features of mixed M1/M2 macrophage polarization with higher expression of CD80+ and CD206+ compared with the residual FSC‐low monocytes and secretion of higher levels of IL‐6, IL‐10, and TNF‐α, when compared with the normal controls. In conclusion, the detection and serial monitoring of this subset of inflammatory monocytes using flow cytometry could be of great help in guiding the prognostication and treatment of patients with COVID‐19 and merits further evaluation.

中文翻译:

前沿科学:COVID-19 感染引起外周血单核细胞易于检测的形态学和炎症相关表型变化

单核细胞/巨噬细胞的过度激活伴随着细胞因子风暴的发展和随后的急性肺损伤,导致急性呼吸窘迫综合征 (ARDS),这是感染 COVID-19 的可怕后果。识别并潜在地早期干预那些最有可能发生这种并发症的患者的能力可能具有很大的临床实用性。在这项研究中,我们在 2020 年初对 34 名 COVID-19 患者的外周血样本进行了流式细胞术分析,试图找出有助于预测疾病严重程度和患者预后的因素。尽管我们没有检测到 COVID-19 患者和正常健康个体之间单核细胞数量的显着差异,但我们确实发现了显着的形态和功能差异,这在需要长期住院和入住重症监护室 (ICU) 的患者中更为明显。COVID-19 患者的单核细胞大于正常单核细胞,通过常规流式细胞术在前向散射 (FSC) 和侧向散射分析中很容易识别,并且存在具有高 FSC (FSC-high) 的独特单核细胞群。在更详细的分析中,这些 CD14+ CD16 +、FSC-高单核细胞表现出混合 M1/M2 巨噬细胞极化的特征,与残留的 FSC-低单核细胞相比,CD80 +和 CD206 +的表达更高,并且分泌更高水平的 IL-6、IL-10 和 TNF -α,与正常对照相比。总之,使用流式细胞术检测和连续监测这部分炎症单核细胞可能对指导 COVID-19 患者的预后和治疗有很大帮助,值得进一步评估。
更新日期:2020-10-11
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