Isostichopus sp. is a variety of Isostichopus badionotus, proposed as a new species, which has been intensely fished in the Colombian Caribbean, arousing interest for its aquaculture. This study evaluated the effect of two culture densities (1–3 larvae ml⁻¹), two temperatures (23°C and 26°C) and two microalgae diets (Isochrysis galbana, Chaetoceros calcitrans, Nannochloropsis oculata 1:1:1 and I. galbana, C. calcitrans 1:1) on the survival, development and growth of its larvae. Larval culture lasted 22 days until metamorphosis to doliolaria larvae, and 7 days later, the first juveniles were observed with a size of 621.8 ± 12.7 µm (±SE). The highest growth rates, survival and percentage of doliolaria larvae were obtained with 1 larva ml⁻¹ (29.2 µm/day and 31.5% doliolaria larvae) and 26°C (28.4 µm/day and 10% doliolaria larvae). However, in the two diets examined, the larvae showed low growth rates (between 1.3 and 8.5 µm/day), stagnation in development and high mortality. Our results indicate that it is feasible to culture larvae from Isostichopus sp. to juveniles, recommending the use of 1 larva ml⁻¹ and 26°C. However, to meet the nutritional needs of the larvae the inclusion of microalgae Pavlova sp. and Tetraselmis chuii in the diet is recommended. This paper reports for the first time the successful production of sea cucumber juveniles of this species in Colombia.

中文翻译：

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密度，温度和日粮对Isostichopus sp。幼虫和幼虫生长，存活和发育的影响。

Isostichopus SP。是Isostichopus badionotus的一种，被提议为新物种，已在哥伦比亚加勒比海地区大量捕捞，引起了对其水产养殖的兴趣。这项研究评估了两种培养密度（1-3幼虫ml ^-1），两种温度（23°C和26°C）和两种微藻饮食（等鞭金藻，南美白对虾，拟南芥，Nannochloropsis oculata 1：1：1和I）的影响。 galbana，C。calcitrans 1：1）对其幼虫的存活，发育和生长。幼虫培养持续22天，直到变态为杜鹃花幼虫为止； 7天后，观察到第一个幼体的大小为621.8±12.7 µm（±SE）。用1个幼虫ml ^-1（29.2 µm /天和31.5％的白毛虫幼虫）和26°C（28.4 µm /天和10％的白毛虫幼虫）可获得最高的生长速率，存活率和白毛虫百分数。但是，在两种日粮中，幼虫的生长速度低（1.3至8.5 µm /天），发育停滞，死亡率高。我们的结果表明，培养来自异骨鱼类的幼虫是可行的。对于幼鱼，建议使用1毫升^-1的幼虫，温度为26°C。然而，为了满足幼虫的营养需要，加入了微藻Pavlova sp。和Tetraselmis chuii在饮食上建议。本文首次报道了该品种的海参幼体在哥伦比亚的成功生产。