Innovation in genomics, transcriptomics, and proteomics research has created a plethora of state‐of‐the‐art techniques such as nucleic acid sequencing and mass‐spectrometry‐based proteomics with paramount impact in the life sciences. While current approaches yield quantitative abundance analysis of biomolecules on an almost routine basis, coupling this high content to spatial information in a single cell and tissue context is challenging. Here, current implementations of spatial omics are discussed and recent developments in the field of DNA‐barcoded fluorescence microscopy are reviewed. Light is shed on the potential of DNA‐based imaging techniques to provide a comprehensive toolbox for spatial genomics and transcriptomics and discuss current challenges, which need to be overcome on the way to spatial proteomics using high‐resolution fluorescence microscopy.

中文翻译：

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用于空间组学的 DNA 条形码荧光显微镜

基因组学、转录组学和蛋白质组学研究的创新创造了大量最先进的技术，例如核酸测序和基于质谱的蛋白质组学，对生命科学产生了重大影响。虽然目前的方法几乎可以常规地对生物分子进行定量丰度分析，但将这种高含量与单个细胞和组织环境中的空间信息相结合是具有挑战性的。在这里，讨论了空间组学的当前实施，并回顾了 DNA 条形码荧光显微镜领域的最新发展。阐明了基于 DNA 的成像技术的潜力，为空间基因组学和转录组学提供了一个综合工具箱，并讨论了当前的挑战，