The enzyme monoamine oxidase B (Mao B), which is involved in dopamine metabolism, is an important drug target in Parkinson’s disease (PD). The medical treatment with several Mao B inhibitors is well established but only little personalized since the monitoring of the patients Mao B activity is complex and requires sophisticated laboratory equipment. To demonstrate an approach, which has potential in the personalization of the medical PD treatment, a sensorial Mao B activity determination system has been developed. Here, the enzyme activity is quantified by electrochemical detection of enzymatically produced H₂O₂. Therefore, the enzyme is enriched from solution via cellulose particles with specific antibodies against human Mao B. The successful capturing of the enzyme can be verified by means of SDS-PAGE. For activity determination the enzyme is brought in contact with a suitable substrate - here benzylamine. Selectivity of the amperometric hydrogen peroxide detection in the presence of co-reactants has been verified. Within the time span of 30 min, a linear dependency of enzymatically produced H₂O₂ with the substrate incubation time can be observed. This allows the evaluation of the Mao B activity. The results have been correlated to an optical detection method. Furthermore, the method has been tested for different amounts of enzyme used in the experiments.

参与多巴胺代谢的单胺氧化酶B（Mao B）是帕金森氏病（PD）的重要药物靶标。几种茂B抑制剂的药物治疗方法已经很成熟，但个性化治疗却很少，这是因为对患者的茂B活性的监测很复杂，需要复杂的实验室设备。为了演示一种在医学PD治疗的个性化方面具有潜力的方法，已经开发了一种感觉毛B活动测定系统。在此，通过电化学检测酶产生的H ₂ O ₂来定量酶活性。。因此，酶通过纤维素颗粒从溶液中富集了针对人毛毛菌B的特异性抗体。可以通过SDS-PAGE验证酶的成功捕获。为了确定活性，使酶与合适的底物-这里是苄胺接触。在共反应物存在下，安培过氧化氢检测的选择性已得到验证。在30分钟的时间内，可以观察到酶促产生的H ₂ O ₂与底物孵育时间的线性关系。这样可以评估毛B的活动。结果已经与光学检测方法相关。此外，该方法已针对实验中使用的不同量的酶进行了测试。