Tissue Cage (TC) model was used to evaluate the pharmacokinetics and ex vivo pharmacodynamics of Minocycline (MINO) after intramuscular (IM) administration to donkeys at 4 mg/kg body-weight. The C_max of MINO with 1.79 and 2.63 μg mL⁻¹ was obtained at 2.96 and 1.41 h in TCF (tissue cage fluid) and plasma respectively. The absorption half-lives (t_1/2ka) of MINO were calculated to be 0.71 h in TCF and 0.32 h in plasma, whereas the elimination half-lives (t_1/2ke) were 10.46 h in TCF and 5.95 h in plasma. The distribution volume (V_d/F) of MINO was estimated to be 1.84 L kg⁻¹ in TCF and 1.28 L kg⁻¹ in plasma. The total clearance (CL_b/F) of MINO was computed as 0.12 and 0.15 L/ (h·kg) in TCF and plasma respectively. The area under the concentration-time curve (AUC) of MINO was 32.77 μg mL⁻¹h in TCF and 25.27 μg mL⁻¹h in plasma, respectively.The ex vivo time-kill curves were established for plasma and TCF samples using Salmonella abortus equi. The MIC and MBC of MINO against salmonella were 0.08 and 0.16 μg mL⁻¹ for plasma, 0.04 and 0.08 μg mL⁻¹ for TCF. The plasma C_max/MIC and AUC/MIC values after IM administration were 32.88 ± 9.87 and 315.88 ± 42.65 h, respectively. The TCF C_max/MIC and AUC/MIC values after IM administration were 44.75 ± 9.32 and 819.25 ± 65.23 h, respectively. The values of T > MIC were approximately >36 h in plasma and > 65 h in TCF. These findings from this study suggest that MINO may be therapeutically effective in diseases of donkeys caused by salmonella when used at a dose of 4 mg/kg IM administration.

使用组织笼（TC）模型评估以4 mg / kg体重对驴进行肌肉（IM）施用后米诺环素（MINO）的药代动力学和离体药效学。在2.96和1.41 h分别在TCF（组织笼液）和血浆中分别获得1.79和2.63μgmL ^-1的MINO的C _max。MINO的吸收半衰期（t _{1 / 2ka}）在TCF中为0.71 h，在血浆中为0.32 h，而消除半衰期（t _{1 / 2ke}）在TCF中为10.46 h，在血浆中为5.95 h。MINO的分布体积（V _d / F）在TCF中估计为1.84 L kg ^-1，在血浆中为1.28 L kg ^-1。总间隙（CL _b在TCF和血浆中，MINO的/ F）分别计算为0.12和0.15 L /（h·kg）。的浓度-时间曲线的MINO（AUC）下面积为32.77微克毫升^-1在TCF和h 25.27微克毫升^-1血浆小时，respectively.The建立用于使用等离子体和TCF样品体外时间-杀菌曲线沙门氏菌马匹流产。MINO的针对沙门氏菌的MIC和MBC分别为0.08和0.16微克毫升^-1为等离子体，0.04和0.08微克毫升^-1为TCF。IM给药后的血浆C _max / MIC和AUC / MIC值分别为32.88±9.87和315.88±42.65 h。TCF C_最大值IM给药后的/ MIC和AUC / MIC值分别为44.75±9.32和819.25±65.23h。T> MIC的值在血浆中约为> 36 h，在TCF中为> 65 h。这项研究的这些发现表明，当以4 mg / kg的剂量IM施用时，MINO在沙门氏菌引起的驴病中可能具有治疗效果。