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Effect of Vitamin E deficiency on spermatogenesis in mice and its similarity to aging
Reproductive Toxicology ( IF 3.3 ) Pub Date : 2020-10-10 , DOI: 10.1016/j.reprotox.2020.10.003
Hirokatsu Saito 1 , Kenshiro Hara 2 , Satoshi Kitajima 1 , Kentaro Tanemura 2
Affiliation  

Vitamin E (VE) plays numerous important roles in mammals because of its antioxidant activity. As a result, VE deficiency (VED) leads to the dysfunction of central nervous, reproductive, and immune systems. However, few studies have reported the effects of VED on the male reproductive system. In this study, we investigated the effects of VED on male reproductive function and examined its relationship to involution in the male reproductive system with aging. We fed a VED or control diet to 4-week-old mice for 12 or 24 weeks. Following the histopathological analysis of reproductive organs, we found seminiferous tubules with exfoliation in the VED groups, and its frequency was significantly increased compared with the controls. Additionally, in the epididymis, a decrease in spermatozoa and an increase in apoptotic germ cells were observed in the VED groups compared with the controls. By Papanicolaou staining, we also found an increase in the proportion of sperm with abnormal morphology in the VED groups compared with the controls. These reproductive effects induced by VED were highly similar to one aspect of those observed in aged mice. Our findings demonstrate that the aging of the male reproductive system may be accelerated because of the impaired in vivo antioxidant capacity induced by VED.



中文翻译:

维生素E缺乏对小鼠精子发生的影响及其与衰老的相似性

由于其抗氧化活性,维生素 E (VE) 在哺乳动物中发挥着许多重要作用。因此,VE 缺乏 (VED) 会导致中枢神经、生殖和免疫系统功能障碍。然而,很少有研究报告 VED 对男性生殖系统的影响。在这项研究中,我们调查了 VED 对男性生殖功能的影响,并检查了其与男性生殖系统退化与衰老的关系。我们给 4 周大的小鼠喂食 VED 或对照饮食 12 或 24 周。通过生殖器官的组织病理学分析,我们发现VED组生精小管有脱落现象,其频率较对照组显着增加。此外,在附睾中,与对照组相比,在 VED 组中观察到精子减少和凋亡生殖细胞增加。通过巴氏染色,我们还发现与对照组相比,VED 组中形态异常的精子比例有所增加。VED 诱导的这些生殖效应与在老年小鼠中观察到的生殖效应高度相似。我们的研究结果表明,男性生殖系统的衰老可能会因为受损而加速VED 诱导的体内抗氧化能力。

更新日期:2020-10-11
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