Xanthomonas oryzae pv. oryzae (Xoo) induces bacterial leaf blight (BLB), is known to be the most devastating disease of rice. The present investigation for the first time explains the antibacterial, anti-biofilm, and antivirulence potential of the simplest allelochemical catechol. Bacterial viability and growth are significantly reducing in catechol treatment. Further study also reveals that catechol also inhibits primary attachment and preformed biofilm of Xoo even at half MIC concentration. The half MIC concentration of catechol also induce a significant decrease in virulence factors like swimming, swarming, exopolysaccharide, and xanthomonadin production. Next, we investigate the possible antibacterial mode of action of catechol against Xoo. Results show that, the catechol caused oxidative stress and targets cell membrane for its antibacterial activity. Whereas, in silico study reveals that, catechol binds with the catalytic domain of XanA protein and this may be consider as a reason for antibiofilm activity of catechol. Moreover, in virulence assay on rice plants, we observe significant decrement in lesion length in catechol and Xoo co-treated rice leaves as compared with only Xoo treated leaves. All the results clearly show, allelochemical catechol to be a potential compound for the antibacterial, anti-biofilm, and antivirulence agent against Xoo and consequently mitigating the BLB disease advancement in rice.

Xanthomonas oryzae pv。稻米（Xoo）引起细菌性叶枯病（BLB），已知是水稻中最具破坏性的疾病。本研究首次解释了最简单的化感化学邻苯二酚的抗菌，抗生物膜和抗毒力潜力。儿茶酚治疗的细菌生存能力和生长显着降低。进一步的研究还表明，即使在一半的MIC浓度下，邻苯二酚也能抑制Xoo的主要附着和预先形成的生物膜。MIC的一半儿茶酚浓度还导致毒力因子的显着降低，如游泳，蜂拥而至，胞外​​多糖和黄嘌呤酮的产生。接下来，我们研究了儿茶酚对Xoo的可能的抗菌作用方式。结果表明，儿茶酚引起氧化应激并以其抗菌活性为靶标细胞膜。鉴于，计算机研究表明，儿茶酚与XanA蛋白的催化结构域结合，这可能被认为是儿茶酚具有抗生物膜活性的原因。此外，在水稻植物的毒力测定中，与仅Xoo处理的叶片相比，我们观察到邻苯二酚和Xoo共同处理的水稻叶片的病灶长度显着减少。所有结果清楚地表明，化感化学邻苯二酚是潜在的化合物，可作为抗Xoo的抗菌剂，抗生物膜剂和抗毒剂，从而减轻水稻中BLB疾病的进展。