Successful start-up of the anaerobic ammonium oxidization (anammox) system is the main challenge for its application. Two anammox biofilm systems with pulse feeding pattern (AN-P) and constant feeding pattern (AN-C) were operated, and the performance of nitrogen removal and microbial interactions among anammox bacteria, heterotrophs, and other microorganisms were investigated. The total nitrogen removal percentage reached 82% (AN-P) and 82.4% (AN-C) in 13 days and 32 days, respectively. AN-P could start up and reach steady state quicker than AN-C. Candidatus Kuenenia was the dominant functional microorganism in both reactors. Heterotrophs (Pseudomonas and Hyphomicrobium) might play important roles in nitrogen and carbon metabolisms. N-decanoyl-DL-homoserine lactone and N-dodecanoyl-DL-homoserine lactone were detected, and acyl homoserine lactone-based quorum sensing system existed in the anammox system. Complex microbial interactions, including competition, cooperation and cross-feeding co-existed in the anammox system and affected system performance.

厌氧铵氧化（anammox）系统的成功启动是其应用的主要挑战。操作了两个具有脉冲进料模式（AN-P）和恒定进料模式（AN-C）的厌氧生物膜系统，并研究了厌氧细菌，异养生物和其他微生物之间的脱氮性能和微生物相互作用。13天和32天总氮去除率分别达到82％（AN-P）和82.4％（AN-C）。AN-P可以比AN-C更快地启动并达到稳态。暂定Kuenenia是两个反应器的主要功能微生物。异养菌（假单胞菌和次生微生物）可能在氮和碳代谢中起重要作用。检测到N-癸酰基-DL-高丝氨酸内酯和N-十二烷酰基-DL-高丝氨酸内酯，并且在厌氧氨氧化系统中存在基于酰基高丝氨酸内酯的群体感应系统。厌氧氨氧化系统中同时存在着复杂的微生物相互作用，包括竞争，合作和交叉喂食，并影响了系统性能。