Emaraviruses are a genus of plant viruses that have been newly described in the past decade. These viruses, some of which are transmitted by eriophyid mites, are important pathogens of cereals, fruits, and ornamental trees worldwide. This study used sequence data for emaraviruses to design new degenerate primer sets that identify an extensive range of known and unknown emaraviruses. Sequence alignment of the amino acid and nucleotide sequences of RNA-dependent RNA polymerases for 11 accessions among nine emaraviruses confirmed the presence of seven conserved motifs (Pre-A, F, A, B, C, D, and E). Subsequently, new degenerate primers were designed based on motifs F, A, and B, which were the most conserved among the seven motifs. Reverse transcription-polymerase chain reaction using these primers detected known emaraviruses more efficiently than previously known primers. These new primers enabled the identification of a partial nucleotide sequence of a putative novel emaravirus from chrysanthemum leaves showing mosaic or yellowish ringspot symptoms known to be associated with eriophyid mites, Paraphytoptus kikus. These sequences were specifically detected from the symptomatic leaves of a chrysanthemum, and the putative emaravirus was tentatively named chrysanthemum mosaic-associated virus.

Emaraviruses 是植物病毒的一个属，在过去十年中被新描述过。这些病毒，其中一些是由绦虫螨传播的，是世界范围内谷物、水果和观赏树木的重要病原体。本研究使用埃马拉病毒的序列数据来设计新的简并引物组，以识别范围广泛的已知和未知埃马拉病毒。9 种 emaravirus 中 11 个加入的 RNA 依赖性 RNA 聚合酶的氨基酸和核苷酸序列的序列比对证实存在七个保守基序（Pre-A、F、A、B、C、D 和 E）。随后，基于基序 F、A 和 B 设计了新的简并引物，这些基序是七个基序中最保守的。使用这些引物的逆转录聚合酶链反应比以前已知的引物更有效地检测已知的埃马拉病毒。这些新的引物能够从菊花叶中鉴定出一种推定的新型 emaravirus 的部分核苷酸序列，该病毒表现出已知与绦虫螨有关的花叶或淡黄色环斑症状，Paraphytoptus kikus。这些序列是从菊花的有症状的叶子中特异性检测到的，假定的埃马拉病毒被暂时命名为菊花花叶相关病毒。