Based on the impact of volatile organic compounds (VOCs) on secondary metabolite pathways, a novel submerged volatile co-culture system was constructed, and the effects of thirteen fungal and bacterial VOCs were investigated on Ganoderma lucidum exopolysaccharides production. The results demonstrated at least a 2.2-fold increase in exopolysaccharide (EPS) specific production yield in 6 days submerged volatile co-culture of G. lucidum with Pleurotus ostreatus. Therefore, P. ostreatus was selected as a variable culture, and the effects of agitation speed, inoculum size, initial pH, and co-culture volume on EPSs production were investigated using a Taguchi L9 orthogonal array. Finally, the highest concentration of EPSs (3.35 ± 0.22 g L-1) was obtained under optimized conditions; initial pH 5.0, inoculum size 10%, 150 rpm, and 3:1 volume ratio of variable culture to main culture.

中文翻译：

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使用新型浸没挥发性共培养系统增强灵芝胞外多糖的产生

基于挥发性有机化合物 (VOCs) 对次级代谢途径的影响，构建了一种新型的浸没挥发性共培养系统，并研究了 13 种真​​菌和细菌 VOCs 对灵芝胞外多糖生产的影响。结果表明，灵芝与平菇的浸没挥发性共培养 6 天后，胞外多糖 (EPS) 比产量至少增加了 2.2 倍。因此，选择 P. ostreatus 作为可变培养物，并使用田口 L9 正交阵列研究搅拌速度、接种物大小、初始 pH 值和共培养体积对 EPSs 生产的影响。最后，在优化条件下获得最高浓度的 EPSs (3.35 ± 0.22 g L-1)；初始 pH 5.0，接种量 10%，150 rpm，和 3：