In situ molecular architecture analysis of organelles and protein assemblies is essential to understanding the role of individual components and their cellular function, and to engineering new molecular functionalities. Through a super-resolution-driven approach, here we characterize the organization of the ciliary basal foot, an appendage of basal bodies whose main role is to provide a point of anchoring to the microtubule cytoskeleton. Quantitative image analysis shows that the basal foot is organized into three main regions linked by elongated coiled-coil proteins, revealing a conserved modular architecture in primary and motile cilia, but showing distinct features reflecting its specialized functions. Using domain-specific BioID proximity labeling and super-resolution imaging, we identify CEP112 as a basal foot protein and other candidate components of this assembly, aiding future investigations on the role of basal foot across different cilia systems.

原位细胞器和蛋白质装配体的分子结构分析对于理解单个组件的作用及其细胞功能以及设计新的分子功能至关重要。通过超分辨率驱动的方法，在这里我们描述了睫状基底脚的组织，它是基体的附肢，其主要作用是为微管细胞骨架提供锚固点。定量图像分析显示，基脚被三个主要区域组成，这些区域由细长的卷曲螺旋蛋白连接，揭示了初级和活动纤毛中保守的模块化结构，但显示出反映其特殊功能的独特特征。使用特定领域的BioID邻近标记和超分辨率成像，