Abstract The diversity and composition of bioaerosols in the atmosphere have been studied in the recent years in multiple places and under very different meteorological conditions. Pollen grains, fungal propagules and bacteria are the most studied airborne biological particles, usually individually, and correlated to environmental factors such as temperature, wind speed, rain, air pollution, etc. to understand their influence. However, the effect of the height on the distribution and composition of these bioaerosols has not been properly described so far. In this research, we analyzed concurrently these three biological particles at four different heights (1.5 m, 130 m, 500 m and 1000 m) by high-throughput DNA sequencing. We observed that the atmosphere contains a homogeneous distribution and composition of biological particles regardless of the altitude tested. Only a minority of the taxa is differently distributed. Therefore, we here deduce that the altitude does not drastically influence the airborne bioparticles distribution and composition under similar meteorological conditions. We also studied the co-occurrence between different bioaerosols, finding new potential relationships.

中文翻译：

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使用高通量 DNA 测序在四个海拔高度分布空气中的花粉、真菌和细菌

摘要 近年来，在多地、不同气象条件下，人们对大气中生物气溶胶的多样性和组成进行了研究。花粉粒、真菌繁殖体和细菌是研究最多的空气传播生物颗粒，通常单独存在，并与温度、风速、雨水、空气污染等环境因素相关联，以了解它们的影响。然而，高度对这些生物气溶胶的分布和组成的影响到目前为止还没有得到适当的描述。在本研究中，我们通过高通量 DNA 测序同时分析了四种不同高度（1.5 m、130 m、500 m 和 1000 m）的这三种生物颗粒。我们观察到，无论测试的高度如何，大气都包含均匀分布和组成的生物粒子。只有少数分类群的分布不同。因此，我们在这里推断，在类似的气象条件下，海拔高度不会显着影响空气中生物颗粒的分布和组成。我们还研究了不同生物气溶胶之间的共存，发现了新的潜在关系。