Introduction

Diabetes mellitus is a multisystem disease. Oxidative stress and nitric oxide isoforms are involved in diabetic pathogenesis. Ferulic acid is a natural substance that is distributed broadly in plants with strong potent properties.

The aim of the research

This research was designed to study the possible protective role of ferulic acid on oxidative stress and different Nitric oxide synthase isoforms (NOS) in the cerebellum of streptozotocin-induced diabetic rats.

Materials and methods

Twenty-four albino male rats were randomly divided into equal four groups: control group, group 2 received ferulic acid orally (10 mg/kg), group 3 diabetic group, group 4 diabetic rats received ferulic acid. After 8 weeks, the left cerebellar hemisphere was taken for tissue homogenate for oxidative markers and real-time PCR for NOS isoforms. Paraffin sections of the right cerebellar hemisphere were stained with cresyl violet, Luxol fast blue and immnunohistochemically stained for neuronal NOS, inducible NOS and endothelial NOS.

Results

Degenerative changes were seen in the cerebella of the diabetic rats with significant elevation of Malondialdehyde, Nitric Oxide, and decrease of Superoxide dismutase levels. nNOS expression decreased and iNOS expression increased significantly. The ferulic acid-treated group showed a reduction of the degenerative changes in the cerebellum with significant improvement in oxidative stress marker, an increase of nNOS expression, and a decrease of iNOS expression.

Conclusions

Ferulic acid improves cerebellar functional and histopathological changes induced by diabetes which can be attributed mainly to its anti-oxidative effect and its ability to modulate NOS isoforms.

中文翻译：

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评价糖尿病白化大鼠小脑一氧化氮合酶表达的调节作用及阿魏酸可能的保护作用

介绍

糖尿病是一种多系统疾病。氧化应激和一氧化氮亚型参与糖尿病发病机制。阿魏酸是一种天然物质，广泛分布于植物中，具有很强的药效。

研究目的

本研究旨在研究阿魏酸对链脲佐菌素诱导的糖尿病大鼠小脑中氧化应激和不同一氧化氮合酶亚型 (NOS) 的可能保护作用。

材料和方法

24只白化雄性大鼠随机分为四组：对照组，第2组口服阿魏酸（10 mg/kg），第3组糖尿病组，第4组糖尿病大鼠服用阿魏酸。8 周后，左小脑半球被用于组织匀浆的氧化标记和实时 PCR 的 NOS 异构体。右小脑半球的石蜡切片用甲酚紫、Luxol 固蓝染色，并对神经元 NOS、诱导型 NOS 和内皮 NOS 进行免疫组织化学染色。

结果

糖尿病大鼠小脑出现退行性变化，丙二醛、一氧化氮显着升高，超氧化物歧化酶水平降低。nNOS 表达减少，iNOS 表达显着增加。阿魏酸治疗组显示小脑退行性变化减少，氧化应激标志物显着改善，nNOS表达增加，iNOS表达减少。

结论

阿魏酸可改善由糖尿病引起的小脑功能和组织病理学变化，这主要归因于其抗氧化作用和调节 NOS 异构体的能力。