Abstract

The homologous anthocyanin2 (AN2) genes were identified and structurally characterized in two cultivars of hot pepper Capsicum chinense with different fruit exocarp coloration (purple and yellow). In the analyzed cultivars, the anthocyanin content and the expression pattern of the CcAN2 gene and anthocyanin pathway structural genes DFR and UFGT, regulated by AN2, were determined in the vegetative and reproductive organs, including fruit tissues at different stages of its development. A positive correlation between transcription levels of CcAN2, CcDFR, and CcUFGT was shown. A direct link between transcription levels of the analyzed genes and the anthocyanin content was observed in fruit peel, but not in the leaves. It was assumed that, in the vegetative tissues of the studied cultivars, the AN2, DFR, and UFGT genes can participate in plant photoprotection, and the visible purple color due to the anthocyanin accumulation may be associated with the regulatory activity of other MBW genes.

中文翻译：

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辣椒中转录因子基因花色素苷2的表达特征及其对花色素苷含量的影响。水果色不同的品种

摘要

在两个辣椒果实颜色不同的果皮（紫色和黄色）的辣椒品种中，鉴定了同源的花青素2（AN2）基因并对其结构进行了表征。在所分析的品种中，确定了营养和生殖器官（包括处于其发育不同阶段的果实组织）中花青素的含量以及由AN2调控的CcAN2基因和花青素途径结构基因DFR和UFGT的花样表达。CcAN2，CcDFR和CcUFGT的转录水平之间呈正相关被显示。在果皮中观察到了被分析基因的转录水平与花色苷含量之间的直接联系，但在叶中却没有。据推测，在所研究品种的营养组织中，AN2，DFR和UFGT基因可以参与植物的光保护作用，并且由于花青素积累而导致的可见紫色可能与其他MBW基因的调节活性有关。