Microencapsulation technology can be used to improve the probiotic viability under stress condition in the human gastrointestinal tract and during storage. The purpose of this study was to evaluate the protective effect of encapsulation materials on the survival of GABA-producing probiotics using alginate containing cassava starch nanocrystals under simulated gastrointestinal conditions and shelf storage. Lactobacillus brevis ST-69, GABA-producing probiotic strain, was isolated from kimchi and encapsulated using emulsion technique. The GABA activity, encapsulation efficiency, morphology, probiotic viability were evaluated. The encapsulation efficiency using emulsion technique was 89.72%. Probiotic encapsulated in alginate-nanocrystalline starch gel capsules showed high survival rate at 94.97% of probiotic cells under simulated gastrointestinal conditions and during long-life storage at 4 °C compared to free cells. Results showed that for improving the viability of probiotics against gastrointestinal and storage conditions, complex materials with nanocrystalline starch might be a better encapsulating matrix for the preparation of gel capsules.

微胶囊技术可用于提高益生菌在人体胃肠道应激条件下和储存过程中的活力。本研究的目的是评估在模拟胃肠道条件和货架储存条件下，使用含有木薯淀粉纳米晶体的海藻酸盐封装材料对产生 GABA 的益生菌的存活的保护作用。短乳杆菌ST-69 是一种产生 GABA 的益生菌菌株，是从泡菜中分离出来的，并使用乳液技术进行胶囊化。评估了 GABA 活性、包封效率、形态、益生菌活力。乳化技术的包封率为89.72%。与游离细胞相比，封装在海藻酸盐纳米晶淀粉凝胶胶囊中的益生菌在模拟胃肠道条件下和 4°C 长期储存期间显示出 94.97% 的益生菌细胞存活率。结果表明，为了提高益生菌在胃肠道和储存条件下的活力，纳米晶淀粉复合材料可能是制备凝胶胶囊的更好的包封基质。