LINC00504 acts as an oncogene and associates with unfavorable prognosis in patients with lung cancer. Silencing LINC00504 may be a promising strategy for treatment of lung cancer and its effects were firstly investigated in lung cancer cells this study. The gene expression level of miR-876-3p as well as LINC00504 were measured via PCR assay. The cell proliferation was investigated through Cell Counting Kit-8 (CCK-8) assay and colony formation assay. Flow cytometry was applied for detection of cell apoptosis. Wound healing and transwell assay were performed for measurement of cell migration and invasion respectively. The apoptosis related protein expressions were measured by western blot. Luciferase report assay was conducted for verification the target gene. LINC00504 was higher expressed in five types of lung cancer cells studied herein when compared with the control normal cells. LINC00504 knockdown exerted inhibitory effects on cell apoptosis, cell migration as well as cell invasion and promoted cell apoptosis. All the effects mentioned above were counteracted by miR-876-3p inhibitor. Silencing LINC00504 possessed anti-proliferation, repression of cell invasion as well as migration and pro-apoptosis effects via targeting up-regulation of miR-876-3p in lung cancer cells, proving the new therapeutic targets and highlighting the potential application in future diagnosis and treatment in lung cancer.

LINC00504 作为癌基因并与肺癌患者的不良预后相关。沉默 LINC00504 可能是治疗肺癌的一种有前景的策略，本研究首次在肺癌细胞中研究了其作用。通过PCR测定测量miR-876-3p以及LINC00504的基因表达水平。通过细胞计数试剂盒-8 (CCK-8) 测定和集落形成测定研究细胞增殖。流式细胞术用于检测细胞凋亡。分别进行伤口愈合和transwell测定以测量细胞迁移和侵袭。Western blot检测凋亡相关蛋白表达。进行荧光素酶报告测定以验证靶基因。与对照正常细胞相比，LINC00504 在本文研究的五种类型的肺癌细胞中表达更高。LINC00504敲低对细胞凋亡、细胞迁移和细胞侵袭具有抑制作用，促进细胞凋亡。上述所有影响都被 miR-876-3p 抑制剂抵消。沉默 LINC00504 通过靶向肺癌细胞中 miR-876-3p 的上调具有抗增殖、抑制细胞侵袭以及迁移和促凋亡作用，证明了新的治疗靶点并突出了其在未来诊断和治疗中的潜在应用肺癌的治疗。上述所有影响都被 miR-876-3p 抑制剂抵消。沉默 LINC00504 通过靶向肺癌细胞中 miR-876-3p 的上调具有抗增殖、抑制细胞侵袭以及迁移和促凋亡作用，证明了新的治疗靶点并突出了其在未来诊断和治疗中的潜在应用肺癌的治疗。上述所有影响都被 miR-876-3p 抑制剂抵消。沉默 LINC00504 通过靶向肺癌细胞中 miR-876-3p 的上调具有抗增殖、抑制细胞侵袭以及迁移和促凋亡作用，证明了新的治疗靶点并突出了其在未来诊断和治疗中的潜在应用肺癌的治疗。