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Environmental pollution reducing strategy for scouring of undegummed sisal fibers using xylanase and pectinase enzymes
Bioprocess and Biosystems Engineering ( IF 3.8 ) Pub Date : 2020-10-08 , DOI: 10.1007/s00449-020-02455-w
Avtar Singh 1, 2 , Libin Mathew Varghese 1 , Bindu Battan 1 , Arun Kumar Patra 3 , Rishi Pal Mandhan 1 , Ritu Mahajan 1
Affiliation  

This study was undertaken to investigate the potential of bioscouring in the processing of undegummed sisal fibers, using xylano-pectinolytic enzymes. Optimum bioscouring was obtained at pH 8.5 and 50 mM buffer molarity, using xylanase (10 IU) and pectinase (8 IU), with a material to liquor proportion of 1:25 (g:ml), EDTA (2 mM) and Tween 80 (0.5%), at 50 °C temperature with agitation rate of 55 rpm and treatment period of 60 min. Enzymatic treatment of sisal fibers enhanced the brightness and whiteness by 11.52 and 6.83%, respectively, and reduced the yellowness by 7.14% in comparison to control. The use of xylanase and pectinase enzymes completely replaced the chemical scouring method for removing non-cellulosic impurities. Thus, enzymatic scouring is energy saving and ecofriendly, since it completely eliminated the use of toxic chemicals used in alkaline scouring. An increase of 23.75% and 11.58% in brightness and whiteness of enzymatically scoured cum bleached fibers, as compared to chemically scoured cum bleached fibers was finally obtained, along with reduction in yellowness by 27.99%. This is the first report demonstrating environmentally sustainable enzymatic approach for scouring of undegummed sisal fibers, using enzymes, simultaneously produced from a bacterial isolate.



中文翻译:

使用木聚糖酶和果胶酶精炼未脱胶剑麻纤维的环境污染减少策略

本研究旨在研究生物煮练在使用木聚糖-果胶分解酶加工未脱胶剑麻纤维中的潜力。在 pH 8.5 和 50 mM 缓冲液摩尔浓度下获得最佳生物煮练,使用木聚糖酶 (10 IU) 和果胶酶 (8 IU),材料与液体的比例为 1:25 (g:ml)、EDTA (2 mM) 和吐温 80 (0.5%),在 50 °C 温度下,搅拌速度为 55 rpm,处理时间为 60 分钟。剑麻纤维的酶促处理与对照相比,亮度和白度分别提高了 11.52% 和 6.83%,黄度降低了 7.14%。木聚糖酶和果胶酶的使用完全取代了去除非纤维素杂质的化学精炼方法。因此,酶法精练既节能又环保,因为它完全消除了碱性煮练中使用的有毒化学品的使用。与化学精练兼漂白纤维相比,酶精练兼漂白纤维的亮度和白度最终分别提高了 23.75% 和 11.58%,同时黄度降低了 27.99%。这是第一份展示环境可持续的酶促方法用于冲刷未脱胶剑麻纤维的报告,使用酶,同时从细菌分离物中产生。

更新日期:2020-10-11
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