The main objective of this work was to assess the cytotoxic activity of Au/Pt/ZnO nanoparticles synthesized using Arctium lappa extract against leukemia. The Au/Pt/ZnO nanoparticles obtained as a result of biological synthesis were characterized by UV-Vis, Scanning (SEM) and Transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and Atomic Force Microscopy (AFM). The applied methods showed that the size of nanoparticles ranged from 10 to 40 nm. This work also assessed the cytotoxicity of Au/Pt/ZnO nanoparticles by means of MTT assay, and analyzed apoptosis as well as the influence of the cultivation time and concentration of Au/Pt/ZnO nanoparticles on the percentage of dead cells. The studies showed that the percentage of dead leukemia cells increased with the cultivation time and concentration of Au/Pt/ZnO nanoparticles. There was observed an increase in the percentage of cells in the G2/M phase, which suggests the stoppage of G2/M leading to cell death. The cytotoxicity of Au/Pt/ZnO nanoparticles determined by means of the MTT test indicated that the viability of leukemia cells practically disappeared when the concentration of the tested nanoparticles was 10 mol.

这项工作的主要目的是评估使用Arctium lappa合成的 Au/Pt/ZnO 纳米粒子的细胞毒活性提取物抗白血病。作为生物合成结果获得的 Au/Pt/ZnO 纳米粒子通过紫外-可见光、扫描 (SEM) 和透射电子显微镜 (TEM)、傅里叶变换红外光谱 (FTIR) 和原子力显微镜 (AFM) 进行表征。所应用的方法表明，纳米粒子的尺寸范围为 10 至 40 nm。本工作还通过 MTT 法评估了 Au/Pt/ZnO 纳米颗粒的细胞毒性，并分析了细胞凋亡以及 Au/Pt/ZnO 纳米颗粒的培养时间和浓度对死细胞百分比的影响。研究表明，随着培养时间和 Au/Pt/ZnO 纳米粒子浓度的增加，死亡白血病细胞的百分比增加。观察到 G2/M 期细胞百分比增加，这表明 G2/M 的停止导致细胞死亡。通过MTT测试测定的Au/Pt/ZnO纳米颗粒的细胞毒性表明，当测试的纳米颗粒浓度为10mol时，白血病细胞的活力几乎消失。