Background: The realization of tissue donations is bound to a tight timeframe. Depending on the type of tissue, time limits are specified within which the donation must be procured and processed. Otherwise, there is a risk of tissue quality loss with increasing time intervals from cardiovascular arrest. According to the European Directorate for the Quality of Medicines and HealthCare (EDQM) guide, cornea must be procured and processed within 72 h after death. The question arises whether this time interval has an influence on the quality of transplanted tissues and how it affects the accomplishment of tissue donations. Methods: In order to obtain information on this, the numbers of tissue donations in the network of the German Society for Tissue Transplantation (DGFG) were evaluated as a function of the death to retrieval time (DRT) as well as the death to preservation time (DPT). For this purpose, 21,454 database entries of cornea donations made in the period from 2014 to 2018 were included. Results: The results show that nearly 50% of donations realized in the DGFG network could be processed only 48 h or later after cardiovascular death due to the opt-in regulation in Germany. For these donations, there seems to be a higher discard rate compared to donations taken earlier. Nevertheless, there is a transplantation rate for these grafts of more than 65%, which is comparable to average transplantation rates stated in the literature. Conclusion: All corneas finally selected for transplantation must meet the specified quality parameters. Since this naturally also applies to transplants that could only be procured at later time points, it can be concluded that DPT up to 72 h for corneal tissue is adequate and has no influence on the quality of corneas that are ultimately transplanted.
Transfus Med Hemother

中文翻译：

---

角膜采购和处理长达 72 小时：角膜移植质量无风险

背景：组织捐赠的实现必然有一个紧迫的时间框架。根据组织的类型，规定了必须采购和处理捐赠的时间限制。否则，随着心血管骤停时间间隔的增加，存在组织质量损失的风险。根据欧洲药品和医疗保健质量理事会 (EDQM) 指南，必须在死后 72 小时内获取和处理角膜。问题是这个时间间隔是否会影响移植组织的质量以及它如何影响组织捐赠的完成。方法：为了获得这方面的信息，德国组织移植协会 (DGFG) 网络中的组织捐赠数量被评估为死亡至恢复时间 (DRT) 以及死亡至保存时间 (DPT) 的函数）。为此，纳入了 2014 年至 2018 年期间进行的 21,454 个角膜捐赠数据库条目。结果：结果表明，由于德国的选择加入规定，在 DGFG 网络中实现的近 50% 的捐赠只能在心血管死亡后 48 小时或更晚的时间内处理。对于这些捐赠，与之前的捐赠相比，丢弃率似乎更高。然而，这些移植物的移植率超过 65%，与文献中所述的平均移植率相当。结论：最终选择移植的所有角膜必须符合规定的质量参数。由于这自然也适用于只能在稍后时间点进行的移植，因此可以得出结论，对角膜组织进行长达 72 小时的 DPT 就足够了，并且对最终移植的角膜质量没有影响。
Transfus Med Hemother