Abstract The article presents research on the exploring of extraction process of biologically active substances from the leaves of the Moringa oleifera tree using ethanol. Ethanolic extracts were obtained using three different techniques: maceration with shaking, ultrasound-assisted extraction and extraction in Soxhlet apparatus, in different time variants: 1, 2 and 4-hours. After solvent evaporating and drying, the yields of dry extracts obtained in particular processes were calculated. The antioxidant activity of extracts was analyzed spectrophotometrically using DPPH radical scavenging method, and total phenolic content (TPC) was determined by Folin-Ciocalteu method. By means of gas chromatography with mass selective detector (GC-MS), 11 biologically active compounds present in ethanolic extracts were identified, among which α-tocopherol had the greatest share. Based on the results, the influence of the extraction technique and time on the yield and antioxidant activity of M. oleifera leaf extracts were discussed.

中文翻译：

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提取工艺对辣木叶提取物得率和抗氧化活性的影响

摘要 本文介绍了利用乙醇从辣木树叶中提取生物活性物质的探索性研究。乙醇提取物是使用三种不同的技术获得的：摇动浸渍、超声辅助提取和索氏装置中的提取，不同的时间变量：1、2 和 4 小时。在溶剂蒸发和干燥后，计算在特定过程中获得的干提取物的产率。提取物的抗氧化活性采用DPPH自由基清除法进行分光光度法分析，总酚含量(TPC)采用Folin-Ciocalteu法测定。通过带有质量选择检测器的气相色谱法 (GC-MS)，鉴定了乙醇提取物中存在的 11 种生物活性化合物，其中α-生育酚所占比例最大。在此基础上，讨论了提取工艺和时间对油茶叶提取物产量和抗氧化活性的影响。