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Fungal pretreatment to enhance the yield of phytochemicals and evaluation of α ‐amylase and α ‐glucosidase inhibition using Cinnamomum zeylanicum (L.) quills pressurized water extracts
Letters in Applied Microbiology ( IF 2.4 ) Pub Date : 2020-11-10 , DOI: 10.1111/lam.13410
W.A.N. Madushika Wariyapperuma 1 , S. Kannangara 2 , Y.S. Wijayasinghe 3 , S. Subramanium 4 , B. Jayawardena 1
Affiliation  

Bioactive compounds entrapped in plant materials can be effectively recovered using fungal enzymes. Cinnamomum zeylanicum Sri Wijaya (SW) and Sri Gemunu (SG) accessions and commercially available C. zeylanicum (CC) were subjected to fungal pretreatment and extracted with pressured water (PWE, 0.098 MPa). Thirteen fungal strains were isolated and the substrate utilization ability of the strains was tested using cellulose, pectin and lignin (indirectly). Total phenolic content (TPC, Folin-Ciocalteu method), proanthocyanidin content (PC, vanillin assay) and α-amylase and α-glucosidase inhibitory potential of the extracts were evaluated. The anti-diabetic drug, Acarbose was used as the positive control. Trichoderma harzianum (MH298760) showed the highest cell lysis ability and hence was used for the microbial pretreatment process. Extracts of SW treated with T. harzianum strain (Pre-SW) gave the highest percentage yield (4.08% ± 0.15%), significantly potent inhibition (p < 0.05) of α-amylase and α-glucosidase activities (IC50 57 ± 8 and 36 ± 8 μg ml-1 respectively), TPC (2.24 ± 0.02 mg gallic acid equivalent g-1 ), and PC (48.2 ± 0.4 mg of catechin equivalent g-1 ) compared to Pre-SG, Pre-CC, and non-treated samples. T. harzianum treatment can enhance the hypoglycemic properties, PC, and TPC of Cinnamon extracts and provide new insights into the recovery of phytochemicals.

中文翻译:

真菌预处理以提高植物化学物质的产量以及使用 Cinnamomum zeylanicum (L.) 刺加压水提取物评估 α-淀粉酶和 α-葡萄糖苷酶抑制

使用真菌酶可以有效地回收植物材料中夹带的生物活性化合物。Cinnamomum zeylanicum Sri Wijaya (SW) 和 Sri Gemunu (SG) 种质和市售 C. zeylanicum (CC) 进行真菌预处理并用加压水 (PWE, 0.098 MPa) 提取。分离出13株真菌菌株,并利用纤维素、果胶和木质素(间接)测试了菌株的底物利用能力。评估了提取物的总酚含量(TPC,Folin-Ciocalteu 方法)、原花青素含量(PC,香草醛测定)以及 α-淀粉酶和α-葡萄糖苷酶抑制潜力。抗糖尿病药物阿卡波糖用作阳性对照。哈茨木霉 (MH298760) 显示出最高的细胞裂解能力,因此用于微生物预处理过程。用哈茨木霉菌株 (Pre-SW) 处理的 SW 提取物的产量百分比最高 (4.08% ± 0.15%),显着有效地抑制 (p < 0.05) α-淀粉酶和 α-葡萄糖苷酶活性 (IC50 57 ± 8 和36 ± 8 μg ml-1)、TPC(2.24 ± 0.02 mg 没食子酸当量 g-1)和 PC(48.2 ± 0.4 mg 儿茶素当量 g-1)与 Pre-SG、Pre-CC 和非-处理过的样品。T. harzianum 处理可以增强肉桂提取物的降血糖特性、PC 和 TPC,并为植物化学物质的回收提供新的见解。与 Pre-SG、Pre-CC 和未处理的样品相比,4mg 儿茶素等效物 g-1)。T. harzianum 处理可以增强肉桂提取物的降血糖特性、PC 和 TPC,并为植物化学物质的回收提供新的见解。与 Pre-SG、Pre-CC 和未处理的样品相比,4mg 儿茶素等效物 g-1)。T. harzianum 处理可以增强肉桂提取物的降血糖特性、PC 和 TPC,并为植物化学物质的回收提供新的见解。
更新日期:2020-11-10
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