Chagas disease is one of the most important insect‐vectored diseases in Brazil. The entomopathogenic fungus Metarhizium anisopliae was evaluated against nymphs and adults of Panstrongylus megistus, Triatoma infestans, and T. sordida. Pathogenicity tests at saturated humidity demonstrated high susceptibility to fungal infection. The shortest estimates of 50% lethal time (LT₅₀) for P. megistus varied from 4.6 (isolate E9) to 4.8 days (genetically modified strain 157p). For T. infestans, the shortest LT₅₀ was 6.3 (E9) and 7.3 days (157p). For T. sordida, the shortest LT₅₀ was 8.0 days (157p). The lethal concentration sufficient to kill 50% of T. infestans (LC₅₀) was 1.9 × 10⁷ conidia/ml for strain 157p. In three chicken coops that were sprayed with M. anisopliae, nymphs especially were well controlled, with a great population reduction of 38.5% after 17 days. Therefore M. anisopliae performed well, controlling Triatominae in both laboratory and field studies.

中文翻译：

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实验室和野外研究，利用真菌Metarhizium anisopliae控制南美锥虫病媒介

恰加斯病是巴西最重要的昆虫媒介疾病之一。昆虫病原真菌Metarhizium anisopliae评估了若虫和成年Panstrongylus megistus，Triatoma infestans和T. sordida的成虫。在饱和湿度下的致病性测试表明，对真菌感染的敏感性很高。棉假单胞菌的50％致死时间的最短估计时间（LT ₅₀）从4.6天（分离E9）到4.8天（转基因菌株157p）不等。对于T. infestans，最短LT ₅₀为6.3（E9）和7.3天（157p）。对于T. sordida而言，最短的LT ₅₀是8.0天（157p）。对于157p菌株，足以杀死50％的T. infestans的致死浓度（LC ₅₀）为1.9×10 ⁷分生孢子/ ml。在这种喷三个鸡舍绿僵菌，若虫尤其是被很好的控制，用17天之后有很大的人口减少了38.5％。因此，anisopliae的表现良好，在实验室和现场研究中均能控制Triatominae。