Osteoporosis is the most prevalent metabolic bone disease and one of the most important postmenopausal consequences. The aim of this study was to investigate the effects of quercetin (Q) and vitamin E (vitE) on ovariectomy‐induced osteoporosis. Animals were ovariectomized and treated with Q (15 mg/kg/day), vitE (60 mg/kg/day), estradiol (10 µg/kg/day), and Q (7.5 mg/kg/day) + vitE (30 mg/kg/day) for 10 weeks by gavage, and osteoporosis markers and messenger RNA (mRNA) expression of autophagy and apoptosis‐related genes were analyzed in serum and tibia of rats. Data indicated that ovariectomy resulted in development of osteoporosis as demonstrated by reduction in serum calcium, bone weight, bone volume, trabeculae volume, and the total number of osteocytes and osteoblasts, and increase in the total number of osteoclasts and serum osteocalcin. Total mRNA expressions of LC3, beclin1, and caspase 3 were also increased and bcl2 expression was decreased in the tibia. By reversing these changes, treatment with Q and vitE markedly improved osteoporosis. In conclusion, Q, and to a lesser extent, vitE, prevented osteoporosis by regulating the total number of bone cells, maybe through regulating autophagy and apoptosis.

中文翻译：

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槲皮素和维生素 E 通过调节大鼠骨细胞的自噬和凋亡减轻卵巢切除引起的骨质疏松症

骨质疏松症是最普遍的代谢性骨病，也是最重要的绝经后后果之一。本研究的目的是研究槲皮素 (Q) 和维生素 E (vitE) 对卵巢切除术引起的骨质疏松症的影响。动物被切除卵巢并用 Q (15 mg/kg/天)、vitE (60 mg/kg/天)、雌二醇 (10 µg/kg/天) 和 Q (7.5 mg/kg/天) + vitE (30 mg/kg/day) 灌胃 10 周，并在大鼠血清和胫骨中分析骨质疏松标志物和自噬和凋亡相关基因的信使 RNA (mRNA) 表达。数据表明，卵巢切除术导致骨质疏松症的发展，如血清钙、骨重量、骨体积、骨小梁体积以及骨细胞和成骨细胞总数的减少以及破骨细胞和血清骨钙素总数的增加所证明的。胫骨中 LC3、beclin1 和 caspase 3 的总 mRNA 表达也增加，而 bcl2 表达降低。通过逆转这些变化，Q 和 vitE 治疗显着改善了骨质疏松症。总之，Q 和在较小程度上的 vitE 通过调节骨细胞总数来预防骨质疏松症，可能是通过调节自噬和细胞凋亡。