The widespread use of mobile phones and Wi‐Fi‐based communication devices makes exposure to radiofrequency electromagnetic fields (RF‐EMF) unavoidable. Previous experiments have revealed the tumor‐promoting effects of non‐ionizing RF‐EMF in adult carcinogen‐treated mice in utero. To extend these investigations, we tested whether these effects are due to the co‐carcinogenicity of RF‐EMF which would manifest as elevated DNA damage. Similar to previous experiments, pregnant mice were exposed to RF‐EMF (Universal Mobile Telecommunication System [UMTS] standard, approximately 1,960 MHz) from day 7 post‐conception (p.c.) at 0 (sham), 0.04, and 0.4 W/kg SAR. At day 14 p.c., the mice were injected with the carcinogen ethylnitrosourea (ENU, 40 mg/kg). At three time‐points specifically 24, 36, and 72 h later, the pregnant females were sacrificed and the fetuses (n = 24–57) were removed. A dye (cy3) specific for adenyl adducts was used to detect DNA damage by fluorescence microscopy in the brain, liver, and lung of each fetus. Compared to control (0 W/kg SAR), exposure to RF‐EMF had no effect on the formation of DNA adducts in the inspected tissues. We conclude that increased adenyl formation of DNA by RF‐EMF exposure is not a valid explanation for the previously reported tumor‐promoting effects of RF‐RMF. Our findings may help to gain a deeper insight into the biological effects of RF‐EMF exposure in the context of malignancy. © 2020 The Authors. Bioelectromagnetics published by Wiley Periodicals LLC on behalf of Bioelectromagnetics Society

中文翻译：

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在用乙基亚硝基脲处理并暴露于 UMTS 射频电磁场的胎鼠的脑、肝和肺中未观察到 DNA 损伤增加

移动电话和基于 Wi-Fi 的通信设备的广泛使用使得暴露于射频电磁场 (RF-EMF) 不可避免。先前的实验已经揭示了非电离 RF-EMF 对子宫内成年致癌物治疗小鼠的肿瘤促进作用。为了扩展这些研究，我们测试了这些影响是否是由于 RF-EMF 的共同致癌性，表现为 DNA 损伤升高。与之前的实验类似，怀孕小鼠从受孕后第 7 天 ( pc .) 以 0（假）、0.04 和 0.4 W/kg暴露于 RF-EMF（通用移动电信系统 [UMTS] 标准，大约 1,960 MHz）特区。在第 14 天pc., 小鼠注射致癌物质乙基亚硝基脲 (ENU, 40 mg/kg)。在三个时间点，特别是 24、36和 72 小时后，处死妊娠雌性并取出胎儿（n = 24-57）。使用对腺苷酸加合物特异的染料 (cy3) 通过荧光显微镜检测每个胎儿的大脑、肝脏和肺中的 DNA 损伤。与对照 (0 W/kg SAR) 相比，暴露于 RF-EMF 对检查组织中 DNA 加合物的形成没有影响。我们得出的结论是，RF-EMF 暴露增加的 DNA 腺苷酸形成并不是之前报道的 RF-RMF 促进肿瘤作用的有效解释。我们的发现可能有助于更深入地了解 RF-EMF 暴露在恶性肿瘤背景下的生物学效应。© 2020 作者。生物电磁学 由 Wiley Periodicals LLC 代表生物电磁学会出版