Indoxyl sulfate (IS) belongs to groups of uremic toxins binding to proteins. This compound may contribute to the generation of oxidative stress in chronic kidney disease (CKD) patients. We hypothesized that a high concentration of IS in the blood may induce structural changes of erythrocyte components and thus may contribute to CKD progression.

In the present study, we evaluated the influence of IS on hemolysate and membrane proteins' conformational state, lipid membrane fluidity, and internal viscosity in erythrocytes. We examined thiols, carbonyl groups, peroxides, and TBARS levels in erythrocyte incubated with IS.

The treatment of erythrocytes with IS led to increase in lipid membrane fluidity, decrease in the internal viscosity of the cells and the motion of the spin labels attached to hemolysate proteins. We did not observe conformational changes in plasma membrane proteins; however, in the plasma membranes of erythrocytes incubated with IS, a decrease in the content of thiol groups and increase in the carbonyls levels and peroxides and TBARS in comparison with the control was observed.

The obtained results indicate that IS induces the oxidative damage of erythrocyte components. This may be an important factor that affects the functional properties of erythrocytes in CKD patients.

硫酸吲哚酚 (IS) 属于与蛋白质结合的尿毒症毒素组。这种化合物可能有助于慢性肾病 (CKD) 患者产生氧化应激。我们假设血液中高浓度的 IS 可能会诱导红细胞成分的结构变化，从而可能导致 CKD 进展。

在本研究中，我们评估了 IS 对红细胞中溶血产物和膜蛋白的构象状态、脂质膜流动性和内部粘度的影响。我们检测了用 IS 孵育的红细胞中的硫醇、羰基、过氧化物和 TBARS 水平。

用 IS 处理红细胞导致脂质膜流动性增加、细胞内部粘度降低以及附着在溶血蛋白上的自旋标记的运动。我们没有观察到质膜蛋白的构象变化；然而，在与 IS 孵育的红细胞的质膜中，与对照相比，观察到硫醇基团含量的减少和羰基水平以及过氧化物和 TBARS 的增加。

所得结果表明IS诱导红细胞成分的氧化损伤。这可能是影响 CKD 患者红细胞功能特性的重要因素。