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FRET-based fluorometry assay for curcumin detecting using PVP-templated Cu NCs
Talanta ( IF 6.1 ) Pub Date : 2020-10-08 , DOI: 10.1016/j.talanta.2020.121741
Zhixia Yao , Hanmeng Liu , Yaosheng Liu , Yongxing Diao , Guangxing Hu , Qifang Zhang , Zhuang Li

A novel fluorometric strategy is proposed for detecting curcumin by polyvinyl pyrrolidone-templated Cu NCs (PVP–Cu NCs) as a fluorescent probe which exhibits excitation/emission peaks at 380/510 nm. The fluorescent excitation and emission spectra of PVP-Cu NCs have a striking overlap with the UV–vis spectrum of curcumin, and the fluorescence lifetime of PVP-Cu NCs decreases after the addition of curcumin. Curcumin leads to fluorescence quenching based on fluorescence resonance energy transfer. This method allows for the determination of curcumin in the range of 0.1–10 μg mL−1 and the detection limit is 21 ng mL−1. Furthermore, this method displays good selectivity and is successfully applied for real sample analysis.



中文翻译:

基于FRET的荧光测定法,使用PVP模板化的Cu NCs检测姜黄素

提出了一种新的荧光测定策略,该方法通过以聚乙烯吡咯烷酮为模板的Cu NCs(PVP–Cu NCs)作为荧光探针检测姜黄素,该荧光探针在380/510 nm处显示激发/发射峰。PVP-Cu NCs的荧光激发和发射光谱与姜黄素的UV-vis光谱显着重叠,添加姜黄素后PVP-Cu NCs的荧光寿命降低。姜黄素基于荧光共振能量转移导致荧光猝灭。该方法可测定姜黄素的范围为0.1–10μgmL -1,检出限为21 ng mL -1。此外,该方法显示出良好的选择性,并成功应用于实际样品分析。

更新日期:2020-10-30
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