It is generally believed that the self-folding of single-stranded DNA depends on the hydrophobic effect of its internal bases, but the folding of a single-stranded DNA in a solution was not disordered and would be affected by the stacking effect of adjacent bases. In this work, we developed a new method to explore the stacking between adjacent bases using Surface-Enhanced Raman Spectroscopy (SERS) for the first time. Acidic titanium ions were introduced into silver nanoparticles as an aggregating agent (Ag@ITNPs), and obtained a symmetrical spectrum by normalizing the peak to deoxyribose at 955 cm⁻¹. Based on the influence of adjacent base stacking on the spectrum, we first identified the point mutation sites accurately by SERS. Also, the base content and the DNA frameshift mutations in ssDNA were precisely analyzed. This new method has a simple experimental process and can accurately capture the changes in the base ring breathing peak intensity caused by different adjacent bases, and thus will provide potential application value in the field of gene diagnosis.

通常认为，单链DNA的自折叠取决于其内部碱基的疏水作用，但是溶液中单链DNA的折叠不会受到干扰，并且会受到相邻碱基的堆积效应的影响。 。在这项工作中，我们首次开发了一种使用表面增强拉曼光谱（SERS）探索相邻碱基之间堆叠的新方法。将酸性钛离子作为聚集剂（Ag @ ITNPs）引入银纳米颗粒中，并通过将955 cm ^-1处的峰归一化为脱氧核糖获得对称光谱。基于相邻碱基堆积对光谱的影响，我们首先通过SERS准确地识别了点突变位点。而且，精确分析了ssDNA中的碱基含量和DNA移码突变。该新方法实验过程简单，可以准确捕获相邻碱基不同引起的基环呼吸峰强度的变化，为基因诊断领域提供潜在的应用价值。