Rural communities in Malaysia have been shown to be exposed to Coxiella, Borrelia and rickettsial infections in previous seroprevalence studies. Further research is necessary to identify the actual causative agents and the potential vectors of these infections. The arthropods parasitizing peri-domestic animals in these communities may serve as the vector in transmitting arthropod-borne and zoonotic agents to the humans.

Molecular screening of bacterial and zoonotic pathogens from ticks and fleas collected from dogs, cats and chickens from six rural communities in Malaysia was undertaken. These communities were made up of mainly the indigenous people of Malaysia, known as the Orang Asli, as well as settlers in oil palm plantations. The presence of Coxiella burnetii, Borrelia, and rickettsial agents, including Rickettsia and Anaplasma, was investigated by performing polymerase chain reaction (PCR) and DNA sequencing.

Candidatus Rickettsia senegalensis was detected in one out of eight pools of Ctenocephalides felis fleas. A relapsing fever group Borrelia sp. was identified from one of seven Haemaphysalis hystricis ticks tested. The results from the PCR screening for Anaplasma unexpectedly revealed the presence of Candidatus Midichloria sp., a potential tick endosymbiont, in two out of fourteen Haemaphysalis wellingtoni ticks tested. C. burnetii was not detected in any of the samples tested.

The findings here provide evidence for the presence of potentially novel strains of rickettsial and borrelial agents in which their impact on public health risks among the rural communities in Malaysia merit further investigation. The detection of a potential endosymbiont of ticks also suggest that the presence of tick endosymbionts in the region is not fully explored.

在先前的血清阳性率研究中，马来西亚的农村社区已显示暴露于柯氏杆菌，疏螺旋体和立克次氏体感染。为了确定这些感染的真正病原体和潜在媒介，有必要做进一步的研究。在这些社区中，寄生于节肢动物周围的节肢动物可作为将节肢动物传播的人畜共患病媒介传播给人类的媒介。

从马来西亚六个农村社区的狗，猫和鸡的tick和蚤中进行细菌和人畜共患病病原体的分子筛查。这些社区主要由马来西亚原住民（称为Orang Asli）以及油棕种植园的定居者组成。的存在贝氏柯克斯体，疏螺旋体属，立克次体和试剂，包括立克次体和无形体，通过进行聚合酶链反应（PCR）和DNA测序分析。

暂定立克次体塞内加尔进一出的8个温泉池的检测栉首蚤跳蚤。复发性发烧组Borrelia sp。从七个被测试的Hyphysalis hystricis壁虱之一确定。从PCR筛选结果无形体意外发现存在暂定Midichloria属，一个潜在的蜱共生菌，进二出十四个长角血wellingtoni蜱测试。在所有测试样品中均未检测到伯氏梭菌。

这里的发现提供了可能存在新的立克次氏菌和雷氏菌病菌株的证据，其中它们对马来西亚农村社区中公共卫生风险的影响值得进一步调查。潜在的壁虱共生菌的检测还表明，该区域内壁虱共生菌的存在尚未得到充分研究。