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Tissue-specific transcriptome recovery on withdrawal from chronic alcohol exposure in zebrafish
Alcohol ( IF 2.3 ) Pub Date : 2020-10-08 , DOI: 10.1016/j.alcohol.2020.10.001
Sofia Banu 1 , Surabhi Srivastava 1 , Arif Mohammed 1 , Gopal Kushawah 1 , Divya Tej Sowpati 1 , Rakesh K Mishra 1
Affiliation  

Alcohol consumption can lead to a wide range of systemic disorders brought about by transcriptional changes. Recent studies have documented altered behavior and physiology in zebrafish exposed to alcohol. In this work, we have identified the changes in the zebrafish transcriptome in response to chronic alcohol exposure. We have further followed the extent of transcriptional recovery upon withdrawal from alcohol and found evidence of tissue-specific responses. Our results indicate a greater extent of recovery of the brain transcriptome compared to the liver. We identify two distinct classes of genes in response to withdrawal from alcohol exposure – those that recover their pre-alcohol expression profile versus those that retain altered expression even after the fish are removed from the alcohol environment. Finally, we have examined gender-specific responses to alcohol exposure in zebrafish and find evidence for distinct alcohol tolerance levels. Upon chronic alcohol exposure, a higher percentage of genes show perturbation in expression profile in males compared to females. Female fish also recover better with more genes regaining the control expression level upon withdrawal from alcohol. Overall, our work identifies genes and pathways perturbed by exposure to alcohol, and demonstrates the extent of gender- and tissue-specific transcriptional changes associated with chronic alcoholism and withdrawal.



中文翻译:

斑马鱼慢性酒精暴露戒断后的组织特异性转录组恢复

饮酒会导致由转录变化引起的多种全身性疾病。最近的研究记录了暴露于酒精的斑马鱼的行为和生理改变。在这项工作中,我们已经确定了斑马鱼转录组响应慢性酒精暴露的变化。我们进一步追踪了戒酒后转录恢复的程度,并发现了组织特异性反应的证据。我们的结果表明,与肝脏相比,大脑转录组的恢复程度更大。我们确定了两种不同类型的基因以响应戒酒——那些恢复其酒精前表达谱的基因与那些即使在将鱼从酒精环境中移除后仍保留改变的表达的基因。最后,我们研究了斑马鱼对酒精暴露的性别特异性反应,并找到了不同酒精耐受水平的证据。在长期酒精暴露后,与女性相比,男性中更高比例的基因表现出表达谱的扰动。随着更多基因在戒酒后恢复控制表达水平,雌性鱼也恢复得更好。总体而言,我们的工作确定了因接触酒精而受到干扰的基因和途径,并证明了与慢性酒精中毒和戒断相关的性别和组织特异性转录变化的程度。随着更多基因在戒酒后恢复控制表达水平,雌性鱼也恢复得更好。总体而言,我们的工作确定了因接触酒精而受到干扰的基因和途径,并证明了与慢性酒精中毒和戒断相关的性别和组织特异性转录变化的程度。随着更多基因在戒酒后恢复控制表达水平,雌性鱼也恢复得更好。总体而言,我们的工作确定了因接触酒精而受到干扰的基因和途径,并证明了与慢性酒精中毒和戒断相关的性别和组织特异性转录变化的程度。

更新日期:2020-11-25
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