A new molecule incorporating two units of 7-nitro-benzoxadiazole (NBD), bridged by m-xylylenediamine, was synthesized and characterized on the basis of analytical and spectroscopic techniques. The metal ion sensing property of this molecule was studied spectroscopically with a large number of metal ions. This study revealed that it can perform as a dual-channel probe for colorimetric as well as fluorometric detection of Hg²⁺. In presence of Hg²⁺, a substantial change in UV-Vis spectrum with the appearance of a new band at 545 nm and a distinct colour change from yellow to red was observed. In the fluorescence spectrum, the intensity of the emission band was substantially quenched only upon addition of Hg²⁺. No significant interference from any other metal ion used in this study was noted, the limit of detection (LOD) for Hg²⁺ was found to be 60 and 10 nM for colorimetric and fluorometric detection method, respectively. This new chemosensor was used for removal of Hg²⁺ from aqueous solution with 92% efficiency. For on-site monitoring and field application, this molecule was immobilized into the agarose based hydrogel film, which was used successfully for detection of Hg²⁺ in water. The study on reversible behaviour of this chemosensor revealed that it can be recycled in solution as well as in solid phase by treatment with Na₂S.

在分析和光谱技术的基础上，合成并结合了一个由两个单元的7-硝基苯并恶二唑（NBD）组成的新分子，该分子被间二甲苯二胺桥接。用大量的金属离子光谱研究了该分子的金属离子感测特性。这项研究表明，它可以用作Hg ^2+的比色和荧光检测的双通道探针。在存在Hg ^2+的情况下，观察到UV-Vis光谱发生了显着变化，出现了545 nm处的新谱带，并且颜色从黄色变为红色。在荧光光谱中，仅在添加Hg ²⁺时，发射带的强度才被淬灭。。没有注意到该研究中使用的任何其他金属离子的显着干扰，对于比色法和荧光法检测方法，发现Hg ^2+的检出限（LOD）分别为60和10 nM。该新型化学传感器用于以92％的效率从水溶液中去除Hg ²⁺。为了进行现场监测和现场应用，将该分子固定在琼脂糖基水凝胶膜中，该膜成功用于检测水中的Hg ²⁺。对这种化学传感器的可逆行为的研究表明，通过用Na ₂ S处理，它既可以在溶液中也可以在固相中再循环。