The study aimed to identify bioactive peptide from Meretrix meretrix Linnaeus foot (MMF) and examine its potential of suppressing inflammation. In brief, the anti-inflammatory activity was identified by erythrocyte membrane protection and protein denaturation assay from MMF peptic 9th-h hydrolysate and was separated with three molecular weight cut-off units. The obtained four fractions were testified for activity and the fraction (10–3 kDa) with maximum activity was purified using gel permeation chromatography. Finally, the peptide sequence was identified as Asn–Pro–Ala–Gln–Asp–Cys (647.559 Da) by liquid chromatography–tandem mass spectrometry (LC–MS/MS). The hexapeptide was characterised for functional properties at different pH range. The non-toxic hexapeptide was able to reduce the cyclooxygenase (COX)-2 activation, pro-inflammatory cytokines and nitric oxide (NO) production significantly in RAW264.7 macrophage cells. The current results propose that the hexapeptide derived from MMF protein can act as an effective anti-inflammatory against pro-inflammatory cytokines, COX-2 and NO. Moreover, it could be used as an effective alternative source for drugs in pharma and also as an ingredient in food industries.

这项研究旨在从Meretrix meretrix中鉴定生物活性肽Linnaeus脚（MMF）并检查其抑制炎症的潜力。简而言之，通过从MMF消化性9h水解物中的红细胞膜保护和蛋白质变性测定鉴定抗炎活性，并用三个截留分子量的单位进行分离。验证获得的四个级分的活性，并使用凝胶渗透色谱法纯化具有最大活性的级分（10–3 kDa）。最后，通过液相色谱-串联质谱法（LC-MS / MS）将肽序列鉴定为Asn-Pro-Ala-Gln-Asp-Cys（647.559 Da）。表征了六肽在不同pH范围内的功能特性。无毒六肽能够显着降低RAW264中的环氧合酶（COX）-2活化，促炎性细胞因子和一氧化氮（NO）的产生。7个巨噬细胞。目前的结果表明，源自MMF蛋白的六肽可以作为针对促炎性细胞因子COX-2和NO的有效抗炎剂。此外，它可以用作制药中药物的有效替代来源，也可以用作食品工业中的成分。