In this research, a novel electrochemical aptasensor for the detection of Penicillin in a milk sample was designed using a Penicillin aptamer as the specific recognition element and an electrospun carbon nanofiber (ECNF) mat electrodeposited gold nanoparticles (AuNPs) as the platform. Firstly, the ECNF mat electrode was fabricated by means of electrospinning and heat treatment method. Secondly, the prepared ECNF mat electrode was modified with electrodeposition of AuNPs to improve the rate of electronic transmission. Finally, a Penicillin aptamer was assembled on the modified electrode. The morphology of AuNPs/ECNF mat electrode was examined using scanning electron microscopy (SEM) equipped with energy dispersive spectroscopy (EDS). The SEM results demonstrated that the electrodeposition of AuNPs on ECNF mat electrode was successfully performed. Cyclic voltammetry (CV) was utilized to indicate the electrochemical performance of the prepared aptasensor. The CV results illustrated a high selectivity, good stability, excellent reproducibility and repeatability as well as wide linear range (1–400 ng/mL) with a low detection limit (0.6 ng/mL). Moreover, the recoveries obtained in this research were in a good agreement with those achieved using the HPLC method. Therefore, a proposed aptasensor with a good electrochemical performance and simple preparation procedure can be proposed for the detection of Penicillin antibiotic in milk samples.

在这项研究中，设计了一种用于检测牛奶样品中青霉素的新型电化学适体传感器，该方法使用青霉素适体作为特异性识别元素，并使用电纺碳纳米纤维（ECNF）垫电沉积的金纳米颗粒（AuNPs）作为平台。首先，通过静电纺丝和热处理方法制备了ECNF毡电极。其次，通过电沉积AuNPs修饰制备的ECNF毡电极，以提高电子传输速率。最后，将青霉素适体装配在修饰的电极上。使用配备了能量色散光谱仪（EDS）的扫描电子显微镜（SEM）检查了AuNPs / ECNF垫电极的形貌。SEM结果表明，成功地在ECNF垫电极上进行了AuNPs的电沉积。利用循环伏安法（CV）指示制备的适体传感器的电化学性能。CV结果显示出高的选择性，良好的稳定性，出色的重现性和可重复性，以及宽的线性范围（1-400 ng / mL）和低检测限（0.6 ng / mL）。此外，在这项研究中获得的回收率与使用HPLC方法获得的回收率非常一致。因此，可以提出一种具有良好的电化学性能和简单的制备过程的适体传感器，用于检测牛奶样品中的青霉素抗生素。出色的重现性和可重复性，线性范围宽（1-400 ng / mL），检测限低（0.6 ng / mL）。此外，在这项研究中获得的回收率与使用HPLC方法获得的回收率非常一致。因此，可以提出一种具有良好的电化学性能和简单的制备过程的适体传感器，用于检测牛奶样品中的青霉素抗生素。出色的重现性和可重复性，线性范围宽（1-400 ng / mL），检测限低（0.6 ng / mL）。此外，在这项研究中获得的回收率与使用HPLC方法获得的回收率非常一致。因此，可以提出一种具有良好的电化学性能和简单的制备过程的适体传感器，用于检测牛奶样品中的青霉素抗生素。