Food autonomy represents an essential challenge for the future Moon Village planned by the European Space Agency. Rearing fish on the moon to provide essential amino acids, omega-3 polyunsaturated fatty acids and vitamin B₁₂ could become a reality using integrated multi-trophic aquaculture (IMTA). The Lunar Hatch programme’s goal is to fertilize farmed fish eggs on Earth that would hatch upon arrival at the lunar base. However, the vibrations from a spacecraft’s launch are an unusual situation for fish eggs and may have a negative impact on them. European sea bass (Dicentrarchus labrax) and meagre (Argyrosomus regius) eggs were exposed to mechanical stresses using standard protocol performed to approve a satellite for space launch. The hatching rate was evaluated after vibration expositions at different hours post fertilization (hpf). An initial series of tests subjecting the eggs to orbital rotation demonstrated their integrity. In subsequent tests, mechanical stressors acting on the Soyuz spacecraft was simulated to test impact on fish eggs. The results showed egg robustness for European sea bass at 35 hpf (one-third of the embryo development) and 83 hpf (two thirds) and of meagre at 14 hpf (one third), with no significant difference in hatching rate compared with an unshaken control batch. European sea bass embryos and potentially other fish species with similar incubation periods (4–6 days) seem to be good candidates to surviving a spacecraft launch. This paper discusses the findings and suggests possible future research avenues.

粮食自主权是欧洲航天局计划的未来月亮村的一项重大挑战。使用综合多营养水产养殖（IMTA），将鱼养在月球上以提供必需的氨基酸，omega-3多不饱和脂肪酸和维生素B ₁₂成为现实。月球孵化计划的目标是使地球上将在到达月球基地时孵化的养殖鱼卵受精。然而，飞船发射的振动对于鱼卵来说是不寻常的情况，并且可能对其产生负面影响。欧洲鲈鱼（Dicentrarchus labrax）和微薄（Argyrosomus regius卵被暴露于机械应力下，使用的标准方案是批准卫星进行太空发射。在受精后的不同小时（hpf）振动暴露后评估孵化率。最初的一系列测试使卵子绕轨道旋转，证明了它们的完整性。在随后的测试中，模拟了对联盟号飞船起作用的机械压力源，以测试对鱼卵的影响。结果表明，欧洲鲈鱼在35 hpf（胚胎发育的三分之一）和83 hpf（三分之二）时的卵稳健性以及14 hpf（三分之一）的微弱卵，与未动摇的孵化率没有显着差异控制批次。欧洲鲈鱼胚胎和潜伏期（4–6天）相似的其他鱼类似乎是在航天器发射中幸存的良好候选者。