To evaluate DNA fragmentation and GMO quantification during soya bean protein concentrate and isolate preparation, genetically modified soya bean event GTS 40-3-2 (Roundup Ready^TM soya bean, RRS) was blended with conventional soya beans at mass percentages of 0.9%, 2%, 3%, 5% and 10%. Qualitative PCR and real-time PCR were used to monitor the taxon-specific lectin and exogenous cp4 epsps target levels in all of the main products and by-products, which has practical significance for RRS labelling threshold and traceability. Along the preparation chain, the majority of DNA was distributed in main products, and the DNA degradation was noticed. From a holistic perspective, the lectin target degraded more than cp4 epsps target during both of the two soya bean proteins preparations. Therefore, the transgenic contents in the final protein products were higher than the actual mass percentages of RRS in raw materials. Our results are beneficial to the improvement of GMO labelling legislation and the protection of consumer rights.

为了评估大豆浓缩蛋白和分离物制备过程中的DNA片段化和GMO定量，将转基因大豆事件GTS 40-3-2（Roundup Ready ^TM大豆，RRS）与传统大豆以质量百分比0.9％，2混合。 ％，3％，5％和10％。使用定性PCR和实时PCR监测所有主要产品和副产物中的分类群特异性凝集素和外源cp4 epsps目标水平，这对于RRS标记阈值和可追溯性具有实际意义。沿着制备链，大多数DNA分布在主要产品中，并且注意到DNA降解。从整体的角度来看，凝集素目标的降解超过cp4 epsps在两种大豆蛋白制备过程中均靶向目标。因此，最终蛋白质产品中的转基因含量高于原料中RRS的实际质量百分比。我们的结果有利于改善转基因生物标签法规和保护消费者权益。