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Vulpeculin: a novel and abundant lipocalin in the urine of the common brushtail possum, Trichosurus vulpecula
Open Biology ( IF 5.8 ) Pub Date : 2020-10-07 , DOI: 10.1098/rsob.200218
Grace M Loxley 1 , David O Hooks 2 , Aristotelis Antonopoulos 3 , Anne Dell 3 , Stuart M Haslam 3 , Wayne L Linklater 4 , Jane L Hurst 5 , Robert J Beynon 1
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Lipocalins are a family of secreted proteins. They are capable of binding small lipophilic compounds and have been extensively studied for their role in chemosignalling in rodent urine. Urine of the common brushtail possum (Trichosurus vulpecula) contains a prominent glycoprotein of 20 kDa, expressed in both sexes. We have isolated this protein and determined its primary sequence by mass spectrometry, including the use of metabolic labelling to resolve the leucine/isoleucine isobaric ambiguity. The protein sequence was identified as a lipocalin, and phylogenetic analysis grouped the protein with other marsupial lipocalin sequences in a phylogenetic clade distinct from established cross-species lipocalin sub-families. The pattern of expression in possum urine and the similarity in sequence and structure to other lipocalins suggests this protein may have a role in brushtail possum chemosignalling.



中文翻译:

Vulpeculin:常见刷尾负鼠 Trichosurus vulpecula 尿液中的一种新型丰富的脂质运载蛋白

脂质运载蛋白是一个分泌蛋白家族。它们能够结合小的亲脂性化合物,并因其在啮齿动物尿液中化学信号传导中的作用而被广泛研究。普通刷尾负鼠的尿液(Trichosurus vulpecula) 包含一个突出的 20 kDa 糖蛋白,在两性中均有表达。我们已经分离了这种蛋白质并通过质谱法确定了它的主要序列,包括使用代谢标记来解决亮氨酸/异亮氨酸同量异位的歧义。该蛋白质序列被鉴定为脂质运载蛋白,系统发育分析将该蛋白质与其他有袋动物脂质运载蛋白序列分组在一个不同于已建立的跨物种脂质运载蛋白亚科的系统发育进化枝中。负鼠尿液中的表达模式以及序列和结构与其他脂质运载蛋白的相似性表明该蛋白质可能在刷尾负鼠化学信号传导中起作用。

更新日期:2020-10-07
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