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Potential of standard strains of Bacillus thuringiensis against the tomato pinworm, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)
Egyptian Journal of Biological Pest Control ( IF 2.4 ) Pub Date : 2020-10-06 , DOI: 10.1186/s41938-020-00326-w
J. Sandeep Kumar , J. Jayaraj , M. Shanthi , M. Theradimani , Balasubramani Venkatasamy , S. Irulandi , S. Prabhu

The tomato pinworm Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) is one of the key pests of tomato worldwide, causing an estimated crop loss of 80 to 100%. This pest has developed resistance to several pesticides due to overuse, resulting in control failures in the field. The use of biological insecticides as Bacillus thuringiensis that expressed insecticidal proteins can be an alternative tool by insecticides to suppress the pest population. Laboratory study investigated the efficacy of standard Bacillus thuringiensis (Bt) strains (4D1, 4D4, 4G1, 4K5 and 4XX4) against T. absoluta. Bioassay was conducted using tomato leaf discs treated with spore crystal lysates prepared from the standard strains, and mortality data was subjected to concentration-mortality probit analysis. The LC50 values for Bt 4D1, Bt 4D4 and Bt 4G1 were 6.10, 6.62 and 8.18 μg/ml for the 2nd instar; 9.90, 10.20 and 11.12 μg/ml for the 3rd instar; and 19.82, 23.16 and 24.54 μg/ml for the 4th instar, respectively, while the Bt 4K5 and Bt 4XX4 were not toxic to T. absoluta. This study suggests that Bt strain 4D1 is effective against different larval instars of the pest and can be used in its management.

中文翻译:

苏云金芽孢杆菌标准菌株对番茄pin虫Tab absoluta(Meyrick)(鳞翅目:opter科)的潜力

番茄pin虫Tab absoluta(Meyrick)(鳞翅目:Gelechiidae)是全世界番茄的主要害虫之一,估计造成80%至100%的作物损失。由于过度使用,该害虫对几种农药产生了抗药性,导致田间控制失灵。表达杀虫蛋白的苏云金芽孢杆菌等生物杀虫剂的使用可以成为杀虫剂抑制害虫种群的另一种工具。实验室研究调查了标准苏云金芽孢杆菌(Bt)菌株(4D1、4D4、4G1、4K5和4XX4)对抗绝对黑麦草的功效。使用经标准菌株制备的孢子晶体裂解物处理的番茄叶片进行生物测定,并对死亡率数据进行浓度-死亡率概率分析。Bt 4D1,Bt 4D4和Bt 4G1的LC50值为6.10、6.62和8。第二龄龄为18μg/ ml; 第三龄期:9.90、10.20和11.12μg/ ml;第4龄幼虫分别为19.82、23.16和24.54μg/ ml,而Bt 4K5和Bt 4XX4对绝对黑麦草无毒。该研究表明,Bt菌株4D1对害虫的不同幼虫有效,可用于其防治。
更新日期:2020-10-07
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