The present study aims to investigate the complete long non-coding RNA (lncRNA) and messenger RNA (mRNA) expression profiles in Intracranial aneurysm (IA) patients and controls by RNA sequencing, which reveals the lncRNA with predictive value for IA risk. The comprehensive lncRNA and mRNA expression profiles were detected by RNA-Seq in human IA walls and superficial temporal arteries (STAs), followed by bioinformatics analyses, such as GO analysis, KEGG pathway analysis, and CNC network construction. Subsequently, qRT-PCR was used to profile the expression levels of selected lncRNA (lncRNA ENST000000576153, lncRNA ENST00000607042, lncRNA ENST00000471220, lncRNA ENST00000478738, lncRNA MALAT1, lncRNA ENST00000508090 and lncRNA ENST00000579688) in 30 (small) or 130 (large) peripheral blood leukocytes, respectively. Multivariate logistic regression was utilized to analyze the effects of lncRNA on IA. Receiver operating characteristic (ROC) curve was further drawn to explore the value of lncRNA in predicting IA. Totally 900 up-regulated and 293 down-regulated lncRNAs, as well as 1297 up-regulated and 831 down-regulated mRNAs were discovered in sequencing. Enrichment analyses revealed that they were actively involved in immune/inflammatory response and cell adhesion/extracellular matrix. Co-expression analysis and further enrichment analyses showed that five candidate lncRNAs might participate in IA’s inflammatory response. Besides, after controlling other conventional risk factors, multivariate logistic regression analysis disclosed that low expression of lncRNA ENST00000607042, lncRNA ENST00000471220, lncRNA ENST00000478738, lncRNA MALAT1 in peripheral blood leukocytes were independent risk factors for IA. LncRNA ENST00000607042 has superior diagnostic value for IA. This study reveals the complete lncRNAs expression profiles in IA. The inflammatory response was closely related to IA. Besides, lncRNA ENST00000607042 might be a novel biomarker for IA risk.

中文翻译：

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基于测序和相关生物信息学分析，探索长非编码RNA表达谱与颅内动脉瘤的关联

本研究旨在通过RNA测序研究颅内动脉瘤（IA）患者和对照中完整的长非编码RNA（lncRNA）和信使RNA（mRNA）表达谱，这揭示了具有预测IA风险价值的lncRNA。通过人IA壁和颞浅动脉（STA）中的RNA-Seq检测到完整的lncRNA和mRNA表达谱，然后进行生物信息学分析，例如GO分析，KEGG通路分析和CNC网络构建。随后，使用qRT-PCR分析了30个（小）或130个（大）外周血白细胞中所选lncRNA（lncRNA ENST000000576153，lncRNA ENST00000607042，lncRNA ENST00000471220，lncRNA ENST00000478738，lncRNA MALAT1，lncRNA ENST00000508090和lncRNA ENST00000579688）的表达水平， 分别。利用多元逻辑回归分析lncRNA对IA的影响。进一步绘制了受体工作特征（ROC）曲线，以探索lncRNA在预测IA中的价值。在测序中共发现900个上调和293个下调的lncRNA，以及1297个上调和831个下调的mRNA。富集分析表明，它们积极参与免疫/炎症反应和细胞粘附/细胞外基质。共表达分析和进一步的富集分析表明，五个候选lncRNAs可能参与了IA的炎症反应。此外，在控制了其他常规风险因素后，多因素logistic回归分析显示，lncRNA ENST00000607042，lncRNA ENST00000471220，lncRNA ENST00000478738，外周血白细胞中的lncRNA MALAT1是IA的独立危险因素。LncRNA ENST00000607042对IA具有卓越的诊断价值。这项研究揭示了IA中完整的lncRNAs表达谱。炎症反应与IA密切相关。此外，lncRNA ENST00000607042可能是IA风险的新型生物标志物。