Despite the fast development of technology in the world, diabetic foot wounds cause deaths and massive economical losses. Diabetes comes first among the reasons of non traumatic foot amputations. To reduce the healing time of these fast progressing wounds, effective wound dressings are in high demand. In our study, sheep small intestinal submucosa (SIS) based biocompatible sponges were prepared after SIS decellularization and their wound healing potential was investigated on full thickness skin defects in a diabetic rat model. The decellularized SIS membranes had no cytotoxic effects on human fibroblasts and supported capillary formation by HUVECs in a fibroblast-HUVEC co-culture. Glutaraldehyde crosslinked sponges of three different compositions were prepared to test in a diabetic rat model: gelatin (GS), gelatin: hyaluronic acid (GS:HA) and gelatin: hyaluronic acid: SIS (GS:HA:SIS). The GS:HA:SIS sponges underwent a 24.8 ± 5.4% weight loss in a 7-day in vitro erosion test. All sponges had a similar Young’s modulus under compression but GS:HA:SIS had the highest (5.00 ± 0.04 kPa). Statistical analyses of histopathological results of a 12-day in vivo experiment revealed no significant difference among the control, GS, GS:HA, and GS:HA:SIS transplanted groups in terms of granulation tissue thickness, collagen deposition, capillary vessel formation, and foreign body reaction (P > 0.05). On the other hand, in the GS:HA:SIS transplanted group 80% of the animals had a complete epidermal regeneration and this was significantly different than the control group (30%, P < 0.05). Preclinical studies revealed that the ECM of sheep small intestinal submucosa can be used as an effective biomaterial in diabetic wound healing.

尽管世界技术发展迅速，但糖尿病足部伤口会导致死亡和巨大的经济损失。在非创伤性足部截肢的原因中，糖尿病居首位。为了减少这些快速发展的伤口的愈合时间，对有效的伤口敷料的需求量很大。在我们的研究中，在 SIS 脱细胞后制备了基于羊小肠黏膜下层 (SIS) 的生物相容性海绵，并在糖尿病大鼠模型的全层皮肤缺损中研究了它们的伤口愈合潜力。脱细胞 SIS 膜对人成纤维细胞没有细胞毒性作用，并支持 HUVEC 在成纤维细胞-HUVEC 共培养中形成毛细血管。制备三种不同成分的戊二醛交联海绵在糖尿病大鼠模型中进行测试：明胶 (GS)、明胶：透明质酸 (GS: HA）和明胶：透明质酸：SIS（GS：HA：SIS）。GS:HA:SIS 海绵在 7 天内重量减轻了 24.8 ± 5.4%体外侵蚀试验。所有海绵在压缩下的杨氏模量相似，但 GS:HA:SIS 最高 (5.00 ± 0.04 kPa)。对为期 12 天的体内实验的组织病理学结果进行统计分析显示，对照组、GS、GS:HA 和 GS:HA:SIS 移植组在肉芽组织厚度、胶原沉积、毛细血管形成和毛细血管形成方面没有显着差异。异物反应（P > 0.05）。另一方面，在 GS:HA:SIS 移植组中，80% 的动物有完全的表皮再生，这与对照组有显着差异（30%，P < 0.05）。临床前研究表明，绵羊小肠黏膜下层的 ECM 可作为糖尿病伤口愈合的有效生物材料。