Adeno-associated viral (AAV) vectors are becoming increasingly popular in basic research as well as in clinical gene therapy. Due to its exceptional resistance against physical and chemical stress, however, the increasing use of AAV in laboratories and clinics around the globe raises safety concerns. Proper decontamination of tools and surfaces based on reliable AAV inactivation is crucial to prevent uncontrolled vector dissemination. Although recommended for AAV decontamination, sodium hypochlorite is not compatible with all surfaces found in the laboratory or clinical environment due to its corrosive nature. We, therefore, compared 0.5% sodium hypochlorite to 0.25% peracetic acid (PAA), a second substance declared effective, and to three less aggressive, commonly available alternative disinfectants 70% ethanol, 1.5% hydrogen peroxide, and 0.45% potassium peroxymonosulfate. The impact of all five disinfectants on virus capsid integrity, viral genome integrity, and infectivity upon different exposure times was tested on AAV2 and AAV5, two serotypes with highly different thermostability. While sodium hypochlorite, potassium peroxymonosulfate, and PAA successfully inactivated AAV2 after 1, 5, and 30 min, respectively, ethanol and hydrogen peroxide did not show significant effects on AAV2 even after exposure for 30 min. For AAV5, only sodium hypochlorite and potassium peroxymonosulfate proved efficient capsid and genome denaturation after incubation for 1 and 30 min, respectively. Consequently, ethanol or hydrogen peroxide should not be considered for routine laboratory or clinical use, while 0.45% potassium peroxymonosulfate and 0.5% sodium hypochlorite represent suitable and broadly effective disinfectants for AAV inactivation.

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基于氧化剂的消毒剂对腺相关病毒载体的灭活

腺相关病毒 (AAV) 载体在基础研究和临床基因治疗中变得越来越流行。然而，由于其对物理和化学压力的卓越抵抗力，AAV 在全球实验室和诊所中的使用越来越多，引发了安全问题。基于可靠的 AAV 灭活对工具和表面进行适当的去污对于防止不受控制的病媒传播至关重要。尽管建议用于 AAV 去污，但由于其腐蚀性，次氯酸钠与实验室或临床环境中发现的所有表面不兼容。因此，我们将 0.5% 的次氯酸钠与 0.25% 的过氧乙酸 (PAA)（另一种被宣布有效的物质）以及三种较不具侵略性的常用替代消毒剂（70% 乙醇、1.5% 过氧化氢和 0）进行了比较。45% 过一硫酸钾。所有五种消毒剂在不同暴露时间下对病毒衣壳完整性、病毒基因组完整性和传染性的影响在 AAV2 和 AAV5 上进行了测试，这两种血清型具有高度不同的热稳定性。虽然次氯酸钠、过一硫酸钾和 PAA 分别在 1、5 和 30 分钟后成功灭活 AAV2，但乙醇和过氧化氢即使在暴露 30 分钟后也没有显示出对 AAV2 的显着影响。对于 AAV5，只有次氯酸钠和过氧单硫酸钾分别在孵育 1 分钟和 30 分钟后证明了有效的衣壳和基因组变性。因此，不应考虑将乙醇或过氧化氢用于常规实验室或临床使用，而 0.45% 过一硫酸钾和 0.