Rolandic epilepsy is one of the most common epileptic syndromes in childhood. We used TMT-based proteomics and bioinformatics analysis to identify the differentially expressed proteins in plasma of children with Rolandic epilepsy. Our aim was to provide a molecular basis for exploring possible mechanisms underlying the pathogenesis of epilepsy. Subjects were divided into two groups (five in each): patients with Rolandic epilepsy as cases and patients with migraine as controls. Total proteins were extracted and quantitatively labeled with TMT, then analyzed using liquid chromatography mass spectrometry. Bioinformatics analysis was used to identify the hub genes. A total of 752 proteins were identified, of which 670 contained quantitative proteins. 217 differentially expressed proteins were identified, 46 of which were only upregulated in more than two groups and 111 of which were only downregulated in more than two groups. Bioinformatics analysis revealed top 10 hub genes in the up- and downregulated groups, respectively. Our study demonstrates that some differentially expressed proteins are associated with epilepsy. Activation of acute-phase or innate immune response and complement and fibrinogen systems and repression of glycolysis, lipoprotein metabolism, and antioxidant activity may play a role in the development of epilepsy.

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基于TMT的罗兰癫痫儿童血浆蛋白质组学分析

罗兰癫痫病是儿童时期最常见的癫痫综合症之一。我们使用基于TMT的蛋白质组学和生物信息学分析来鉴定Rolandic癫痫患儿血浆中差异表达的蛋白质。我们的目的是为探索癫痫发病机制的潜在机制提供分子基础。将受试者分为两组（每组五组）：罗兰癫痫患者作为对照组，偏头痛患者作为对照组。提取总蛋白并用TMT定量标记，然后使用液相色谱质谱法进行分析。生物信息学分析用于鉴定中心基因。总共鉴定出752种蛋白质，其中670种包含定量蛋白质。鉴定出217种差异表达的蛋白质，其中只有46个在两个以上的组中被上调，其中111个仅在两个以上的组中被下调。生物信息学分析揭示了分别在上调和下调组中的前10个中心基因。我们的研究表明，一些差异表达的蛋白与癫痫有关。急性期或先天性免疫应答，补体和纤维蛋白原系统的激活以及糖酵解，脂蛋白代谢和抗氧化活性的抑制可能在癫痫的发展中起作用。