The establishment of the beneficial interactions between the host and its microbiota is essential for the correct functioning of the organism, since microflora alterations can lead to many diseases. Probiotics improve balanced microbial communities, exerting substantial health-promoting effects. Here we monitored the molecular outcomes, obtained by gut microflora modulation through probiotic treatment, on human urine and serum metabolic profiles, with a metabolomic approach. Twenty-two subjects were enrolled in the study and administered with two different probiotic types, both singularly and in combination, for 8 weeks. Urine and serum samples were collected before and during the supplementation and were analyzed by nuclear magnetic resonance (NMR) spectroscopy and statistical analyses. After eight weeks of treatment, probiotics deeply influence the urinary metabolic profiles of the volunteers, without significantly altering their single phenotypes. Anyway, bacteria supplementation tends to reduce the differences in metabolic phenotypes among individuals. Overall, the effects are recipient-dependent, and in some individuals, robust effects are already well visible after four weeks. Modifications in metabolite levels, attributable to each type of probiotic administration, were also monitored. Metabolomic analysis of biofluids turns out to be a powerful technique to monitor the dynamic interactions between the microflora and the host, and the individual response to probiotic assumption.

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施用益生菌对人体代谢表型的影响

在宿主及其微生物群之间建立有益的相互作用对于生物体的正确功能至关重要，因为微生物区系的改变会导致许多疾病。益生菌改善了平衡的微生物群落，发挥了显着的健康促进作用。在这里，我们通过代谢组学方法监测了通过益生菌治疗通过肠道菌群调节获得的分子结果，其对人的尿液和血清代谢谱有影响。22名受试者入选了该研究，并分别服用了两种不同类型的益生菌（单独或组合使用），持续8周。在补充之前和期间收集尿液和血清样品，并通过核磁共振（NMR）光谱和统计分析进行分析。经过八周的治疗，益生菌会深深影响志愿者的尿代谢谱，而不会显着改变其单一表型。无论如何，细菌补充趋于减少个体之间代谢表型的差异。总体而言，这些影响是受者依赖的，并且在某些人中，四周后就已经明显看到了强烈的影响。还监测了可归因于每种益生菌施用类型的代谢物水平的变化。生物流体的代谢组学分析已成为监测微生物群与宿主之间动态相互作用以及对益生菌假设的个体反应的一项强大技术。补充细菌往往会减少个体之间代谢表型的差异。总体而言，这些影响是受者依赖的，并且在某些人中，四周后就已经明显看到了强烈的影响。还监测了可归因于每种益生菌施用类型的代谢物水平的变化。生物流体的代谢组学分析已成为监测微生物群与宿主之间动态相互作用以及对益生菌假设的个体反应的一项强大技术。补充细菌往往会减少个体之间代谢表型的差异。总体而言，这些影响是受者依赖的，并且在某些人中，四周后就已经明显看到了强烈的影响。还监测了可归因于每种益生菌施用类型的代谢物水平的变化。生物流体的代谢组学分析已成为监测微生物群与宿主之间动态相互作用以及对益生菌假设的个体反应的一项强大技术。