Background—Medicinal plants are traditionally used as infusions or decoctions for their antioxidant, anti-inflammatory, hypolipidemic and anti-diabetic properties. Purpose—The aim of the study was to define the polyphenol composition and to assess the antioxidant capacity of eight medicinal plants from Reunion Island referred to in the French Pharmacopeia, namely Aphloia theiformis, Ayapana triplinervis, Dodonaea viscosa, Hubertia ambavilla, Hypericum lanceolatum, Pelargonium x graveolens, Psiloxylon mauritianum and Syzygium cumini. Methods—Polyphenol content was assessed by biochemical assay and liquid chromatography coupled to mass spectrometry. Antioxidant capacity was assessed by measuring DPPH reduction and studying the protective effects of herbal preparation on red blood cells or preadipocytes exposed to oxidative stress. Results—Polyphenol content ranged from 25 to 143 mg gallic acid equivalent (GAE)/L for infusions and 35 to 205 mg GAE/L for decoctions. Liquid chromatography coupled to mass spectrometry analysis showed the presence of major bioactive polyphenols, such as quercetin, chlorogenic acid, procyanidin and mangiferin. Antioxidant capacity assessed by different tests, including DPPH and Human red blood cell (RBC) hemolysis of herbal preparations, demonstrated a dose-dependent effect whatever the extraction procedure. Our data suggest that decoction slightly improved polyphenol extraction as well as antioxidant capacity relative to the infusion mode of extraction (DPPH test). However, infusions displayed a better protective effect against oxidative stress-induced RBC hemolysis. Conclusion—Traditional preparations of medicinal plant aqueous extracts (infusions and decoctions) display antioxidant properties that limit oxidative stress in preadipocytes and red blood cells, supporting their use in the context of metabolic disease prevention and treatment.

中文翻译：

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留尼汪岛八种药用植物的水提取物的多酚含量和抗氧化能力的评估：防御红细胞和前脂肪细胞的氧化应激

背景—药用植物由于其抗氧化，抗炎，降血脂和抗糖尿病特性，传统上被用作输液或汤剂。目的—研究的目的是确定多酚的成分并评估法国药典中提到的来自留尼汪岛的八种药用植物，即Aphloia theiformis，Ayapana triplinervis，Dodonaea viscosa，Hubertia ambavilla，Shypericum lanceolatum，天竺葵X天竺葵，Psiloxylon mauritianum和海南蒲桃。方法—通过生化分析和液相色谱-质谱联用对多酚含量进行评估。通过测量DPPH降低并研究草药制剂对暴露于氧化应激的红细胞或前脂肪细胞的保护作用来评估抗氧化能力。结果-输液中多酚含量为25至143 mg没食子酸当量（GAE）/ L，煎剂中多酚含量为35至205 mg GAE / L。液相色谱与质谱分析联用显示，存在主要的生物活性多酚，例如槲皮素，绿原酸，原花青素和芒果苷。通过不同的测试（包括DPPH和人红细胞（RBC）草药制剂的溶血）评估了抗氧化能力，无论采用何种提取程序，均显示出剂量依赖性。我们的数据表明，相对于浸提液的浸提方式（DPPH测试），水煎液可稍微改善多酚的浸提以及抗氧化能力。然而，输注显示出更好的针对氧化应激引起的RBC溶血的保护作用。结论—药用植物水提取物的传统制剂（输液和汤剂）显示出抗氧化特性，可限制前脂肪细胞和红细胞的氧化应激，支持它们在代谢性疾病预防和治疗中的应用。