Emerging evidence indicates the clinical benefits of photobiomodulation therapy (PBMT) in the management of skin and mucosal wounds. Here, we decided to explore the effects of different regiments of PBMT on epithelial cells and stem cells, and the potential implications over the epigenetic circuitry during healing. Scratch‐wound migration, immunofluorescence (anti‐acetyl‐Histone H3, anti‐acetyl‐CBP/p300 and anti‐BMI1), nuclear morphometry and western blotting (anti‐Phospho‐S6, anti‐methyl‐CpG binding domain protein 2 [MBD2]) were performed. Epithelial stem cells were identified by the aldehyde dehydrogenase enzymatic levels and sphere‐forming assay. We observed that PBMT‐induced accelerated epithelial migration and chromatin relaxation along with increased levels of histones acetylation, the transcription cofactors CBP/p300 and mammalian target of rapamycin. We further observed a reduction of the transcription repression‐associated protein MBD2 and a reduced number of epithelial stem cells and spheres. In this study, we showed that PBMT could induce epigenetic modifications of epithelial cells and control stem cell fate, leading to an accelerated healing phenotype.

中文翻译：

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光生物调节疗法驱动大量表观遗传组蛋白修饰、干细胞动员和加速上皮愈合

新出现的证据表明光生物调节疗法 (PBMT) 在治疗皮肤和粘膜伤口方面的临床益处。在这里，我们决定探索不同的 PBMT 团对上皮细胞和干细胞的影响，以及在愈合过程中对表观遗传回路的潜在影响。划痕迁移、免疫荧光（抗乙酰-组蛋白 H3、抗乙酰-CBP/p300 和抗 BMI1）、核形态学和蛋白质印迹（抗-Phospho-S6、抗甲基-CpG 结合域蛋白 2 [MBD2 ]) 进行。通过醛脱氢酶酶水平和成球测定法鉴定上皮干细胞。我们观察到 PBMT 诱导的加速上皮迁移和染色质松弛以及组蛋白乙酰化水平的增加，转录辅助因子 CBP/p300 和雷帕霉素的哺乳动物靶标。我们进一步观察到转录抑制相关蛋白 MBD2 的减少以及上皮干细胞和球体的数量减少。在这项研究中，我们发现 PBMT 可以诱导上皮细胞的表观遗传修饰并控制干细胞的命运，从而导致加速愈合表型。