Ex vivo confocal laser scanning microscopy (CLSM) provides rapid, high‐resolution imaging, fluorescence detection and digital haematoxylin–eosin (H&E)‐like staining. We aimed to assess the performance of ex vivo CLSM in identifying histomorphology and immunoreactivity in lichen planus (LP) and comparing its accuracy with conventional histopathology and direct immunofluorescence (DIF). Thirty‐three sections of 17 LP patients stained with acridine orange (AO) and FITC‐labelled anti‐fibrinogen antibody and 21 control samples stained with AO were examined using ex vivo CLSM. Ex vivo CLSM was in perfect agreement with conventional histopathology in identifying interface dermatitis, vacuolar degeneration and band‐like infiltration. ROC analysis showed that the presence of vacuolar degeneration, interface dermatitis and band‐like infiltration was useful to distinguish LP sections from controls (p < .0001). The detection rates of fibrinogen deposition using DIF and in conclusion ex vivo CLSM were 93.8% and 62.5%, respectively. ex vivo CLSM enables histopathological and immunofluorescence examination in LP with the advantage of digital H&E‐like staining.

中文翻译：

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使用离体共聚焦激光扫描显微镜对扁平苔藓进行免疫荧光和组织病理学评估

离体共聚焦激光扫描显微镜（CLSM）提供快速，高分辨率的成像，荧光检测和数字苏木精-伊红（H＆E）染色。我们旨在评估离体CLSM在鉴定扁平苔藓（LP）的组织形态和免疫反应性方面的性能，并将其与常规组织病理学和直接免疫荧光（DIF）的准确性进行比较。使用离体CLSM检查了17例被a啶橙（AO）和FITC标记的抗纤维蛋白原抗体染色的LP患者的33个切片，以及21个被AO染色的对照样品。离体CLSM与常规组织病理学在确定界面皮炎，液泡变性和条带状浸润方面完全吻合。ROC分析表明存在液泡变性，p  <.0001）。使用DIF和体外CLSM得出的纤维蛋白原沉积的检出率分别为93.8％和62.5％。离体CLSM借助数字H＆E样染色的优势，可以在LP中进行组织病理学和免疫荧光检查。