The application of natural preservatives has become an attractive method for controlling postharvest decay of fruits and vegetables. Allicin, the main active ingredient of allium plants, has broad‐spectrum antifungal activity. However, the unstable properties of allicin limit its wide application. In this study, 1‐[(R)‐ethylsulfinyl]sulfanylethane, a structurally stable derivative of allicin, was used to explore its antifungal activity and potential mechanism on the expansion of Penicillium expansum. We demonstrated the antifungal activity of 1‐[(R)‐ethylsulfinyl]sulfanylethane through in vitro antifungal experiments. At the concentration is 2 mg/L, 1‐[((R)‐ethylsulfinyl]sulfanyl]ethane can completely inhibit spore germination and mycelial growth, whereas the concentration of allicin needs to reach 16 mg/L. Fungal Biochemical assay indicated that decrease of mitochondrial membrane potential, overgeneration of reactive oxygen species, decrease of adenosine triphosphate and glutathione content, increase of superoxide dismutase, catalase, and malondialdehyde content. The results revealed that 1‐[(R)‐ethylsulfinyl]sulfanylethane induced mitochondrial dysfunction and oxidative stress in P. expansum. Due to its excellent antifungal activity, 1‐[((R)‐ethylsulfinyl]sulfanyl]ethane might be developed as a substitute for fungicides against P. expansum in postharvest fruit storage.

中文翻译：

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大蒜素衍生物通过诱导氧化应激对膨胀青霉的抗真菌活性

天然防腐剂的应用已成为控制水果和蔬菜采后腐烂的一种有吸引力的方法。大蒜素是葱属植物的主要活性成分，具有广谱抗真菌活性。然而，大蒜素的不稳定特性限制了其广泛应用。在这项研究中，1-[(R)-乙基亚磺酰基]硫烷基乙烷是一种结构稳定的大蒜素衍生物，用于探索其抗真菌活性和扩大青霉菌的潜在机制。我们通过体外抗真菌实验证明了 1-[(R)-乙基亚磺酰基]硫烷基乙烷的抗真菌活性。在浓度为2mg/L时，1-[((R)-乙基亚磺酰基]硫烷基]乙烷可以完全抑制孢子萌发和菌丝生长，而大蒜素的浓度需要达到16mg/L。真菌生化检测显示线粒体膜电位降低，活性氧过度生成，三磷酸腺苷和谷胱甘肽含量降低，超氧化物歧化酶、过氧化氢酶和丙二醛含量升高。结果表明，1-[(R)-乙基亚磺酰基]硫烷基乙烷在 P. expansum 中诱导线粒体功能障碍和氧化应激。由于其优异的抗真菌活性，1-[((R)-乙基亚磺酰基]硫烷基]乙烷可能被开发作为果实采后贮藏中抗扩展紫菜的杀菌剂的替代品。结果表明，1-[(R)-乙基亚磺酰基]硫烷基乙烷在 P. expansum 中诱导线粒体功能障碍和氧化应激。由于其优异的抗真菌活性，1-[((R)-乙基亚磺酰基]硫烷基]乙烷可能被开发作为果实采后贮藏中抗扩展紫菜的杀菌剂的替代品。结果表明，1-[(R)-乙基亚磺酰基]硫烷基乙烷在 P. expansum 中诱导线粒体功能障碍和氧化应激。由于其优异的抗真菌活性，1-[((R)-乙基亚磺酰基]硫烷基]乙烷可能被开发作为果实采后贮藏中抗扩展紫菜的杀菌剂的替代品。