Rosmarinic acid (RA), an ester compound of caffeic acid (CA) and 3,4‐dihydroxyphenyllacic acid, is widely distributed in the herbs of the Lamiaceae family and has shown a wide spectrum of pharmacological properties. CA and FA (ferulic acid) are two bioactive metabolites in vivo after oral administration of RA; however, a rapid and robust analytical approach that can enable the quantitative assay of RA and two bioactive metabolites is still lacking. A liquid chromatography/tandem mass spectrometry method was established that was capable of the quantitative determination of RA, CA and FA by negative‐mode multiple reaction monitoring within 7 min using a Zorbax SB‐C₁₈ column and an isocratic elution. This assay method was validated as linear over the investigated ranges with correlation coefficients (r) > 0.9950. The intra‐ and inter‐day precision was <10.65%, and the accuracies (relative error, %) <−6.41%. The validated approach was applied to a pharmacokinetics study of RA and its two metabolites in rats after oral and intravenous administration. RA was rapidly metabolized in both administration modes, whilst the metabolites CA and FA were only detectable by oral administration. The absolute availability of RA was calculated to be 4.13%.

中文翻译：

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LC-MS / MS快速测定大鼠血浆中迷迭香酸及其两种生物活性代谢物及其在药代动力学研究中的应用

迷迭香酸（RA）是咖啡酸（CA）和3,4-二羟基苯乙酸的酯化合物，广泛分布于唇形科（Lamiaceae）的草药中，并具有广泛的药理特性。口服RA后，CA和FA（阿魏酸）是体内的两种生物活性代谢物。但是，仍然缺乏能够对RA和两种生物活性代谢物进行定量分析的快速而可靠的分析方法。建立了一种液相色谱/串联质谱方法，该方法能够使用Zorbax SB-C ₁₈在7分钟内通过负模式多反应监测来定量测定RA，CA和FA柱和等度洗脱。该分析方法在相关系数（r）> 0.9950的研究范围内被验证为线性。日内和日间精确度<10.65％，准确度（相对误差，％）<-6.41％。经验证的方法可用于大鼠及其口服和静脉内给药后RA及其两种代谢产物的药代动力学研究。RA在两种给药方式中均被快速代谢，而CA和FA的代谢物仅可通过口服给药检测到。RA的绝对可用性经计算为4.13％。