In this work, the vinyl groups were introduced onto gelatin to create methacrylated gelatin nanoparticles (MA-GNP) as crosslinking centers for fabricating macromolecular microsphere composite (MMC) hydrogels. The MA-GNPs with an average size of ~100 nm, measured with dynamic light scattering, were also visualized using AFM. The presence of CC bonds in the nanoparticles was further confirmed with the proton NMR. Then, the MA-GNP was incorporated as a cross-linker to synthesize MMC hydrogels using acrylamide as the monomer. The resulting hydrogels are porous with tunable pore sizes; and exhibit the improved compressive resistance and elasticity. Increasing the dosage of MA-GNP appropriately led to the reduction of the equilibrium swelling ratio but improved thermal stability of the gels. Moreover, the MA-GNP can endow the gels with enhanced biocompatibility. Overall, the novel protein-based nanospheres are of great potential as both nano-crosslinker and reinforcer to create polymer hydrogels with tailored properties.

在这项工作中，乙烯基被引入到明胶上以生成甲基丙烯酸明胶纳米颗粒（MA-GNP）作为交联中心，用于制造高分子微球复合材料（MMC）水凝胶。使用动态光散射法测量的平均大小约为100 nm的MA-GNP也可以使用AFM进行可视化。C的存在质子NMR进一步证实了纳米颗粒中的C键。然后，掺入MA-GNP作为交联剂以使用丙烯酰胺作为单体合成MMC水凝胶。所得的水凝胶是多孔的，孔径可调。并表现出改善的抗压性和弹性。适当地增加MA-GNP的剂量导致平衡溶胀率的降低，但改善了凝胶的热稳定性。而且，MA-GNP可以赋予凝胶增强的生物相容性。总体而言，基于蛋白质的新型纳米球作为纳米交联剂和增强剂，具有巨大潜力，可制造出具有定制性能的聚合物水凝胶。