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Enterococcus faecium NCIMB10415 responds to norepinephrine by altering protein profiles and phenotypic characters
Journal of Proteomics ( IF 3.3 ) Pub Date : 2020-10-07 , DOI: 10.1016/j.jprot.2020.104003
R Scardaci 1 , F Varese 1 , M Manfredi 2 , E Marengo 2 , R Mazzoli 1 , E Pessione 1
Affiliation  

The long-term established symbiosis between gut microbiota and humans is based upon a dynamic equilibrium that, if unbalanced, could lead to the development of diseases. Despite the huge amount of data concerning the microbiota-gut-brain-axis, little information is available on what happens at the molecular level in bacteria, when exposed to human signals. In the present study, the physiological effects exerted by norepinephrine (NE), a human hormone present in significant amounts in the host gut, were analyzed using the commensal/probiotic strain Enterococcus faecium NCIMB10415 as a target. The aim was to compare the protein profiles of treated and untreated bacteria and relating these proteome patterns to some phenotypic modifications important for bacteria-host interaction. Actually, to date, only pathogens have been considered. Combining a gel-free/label-free proteomic analysis with the evaluation of bile salts resistance, biofilm formation and autoaggregation ability (as well as with the bacterial growth kinetics), allowed to detect changes induced by NE treatment on all the tested probiotic properties. Furthermore, exposure to the bioactive molecule increased the abundance of proteins related to stress response and to host-microbe interaction, such as moonlight proteins involved in adhesion and immune stimulation. The results of this investigation demonstrated that, not only pathogens, but also commensal gut bacteria are affected by host-derived hormones, underlining the importance of a correct cross-signalling in the maintenance of gut homeostasis.

Significance

The crucial role played by the human gut microbiota in ensuring host homeostasis and health is definitively ascertained as suggested by the holobiome concept. The present research was intended to shed light on the endocrinological perturbations possibly affecting microbiota. The microbial model used in this study belongs to Enterococcus faecium species, whose controversial role as gut commensal and opportunistic pathogen in the gut ecosystem is well recognized. The results obtained in the present investigation clearly demonstrate that E. faecium NCIMB10415 can sense and respond to norepinephrine, a human hormone abundant at the gut level, by changing protein profiles and physiology, inducing changes that could favor survival and colonization of the host tissues. To our knowledge, this is the first proteomic report concerning the impact of a human hormone on a commensal/probiotic bacterium, since previous research has focused on exploring the effects of neuroendocrine molecules on growth and virulence of pathogenic species.



中文翻译:

粪肠球菌NCIMB10415通过改变蛋白质谱和表型特征对去甲肾上腺素作出反应

肠道菌群与人类之间建立的长期共生关系基于动态平衡,如果不平衡,则可能导致疾病的发展。尽管有大量关于微生物-肠道-脑轴的数据,但是当暴露于人类信号时,关于细菌分子水平发生的变化的信息很少。在本研究中,使用共生/益生菌粪肠球菌对去甲肾上腺素(NE)(宿主肠道中大量存在的人类激素)发挥的生理作用进行了分析。NCIMB10415作为目标。目的是比较处理过的细菌和未处理过的细菌的蛋白质谱,并将这些蛋白质组模式与对细菌-宿主相互作用很重要的某些表型修饰联系起来。实际上,迄今为止,仅考虑了病原体。将无凝胶/无标记的蛋白质组学分析与胆汁盐抗性,生物膜形成和自动聚集能力(以及细菌生长动力学)的评估相结合,可以检测NE处理对所有测试益生菌性质的诱导变化。此外,暴露于生物活性分子会增加与应激反应和宿主-微生物相互作用有关的蛋白质的丰度,例如参与粘附和免疫刺激的月光蛋白质。这项调查的结果表明,不仅病原体,

意义

完整的概念确定了人类肠道菌群在确保宿主体内稳态和健康中所起的关键作用。本研究旨在阐明可能影响微生物群的内分泌扰动。这项研究中使用的微生物模型属于粪肠球菌,其在肠道生态系统中作为肠道共生和机会病原体的争议作用广为人知。在本次调查中获得的结果清楚地表明粪肠球菌NCIMB10415可以通过改变蛋白质谱和生理结构,诱导可能有利于宿主组织存活和定殖的变化,来感知和响应去甲肾上腺素(一种在肠道水平上丰富的人激素)并做出反应。据我们所知,这是有关人类激素对共生/益生菌细菌影响的第一份蛋白质组学报告,因为先前的研究集中于探讨神经内分泌分子对病原体生长和毒力的影响。

更新日期:2020-10-13
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