Spinal cord injury (SCI) as a crippling disability causes tissue degeneration via neuron loss and fiber disruption. Some researchers have tried to reverse or minimize these changes. Platelet-rich plasma (PRP) is a biological product derived from peripheral blood containing a variety of growth factors. PRP has been extensively used in regenerative medicine. On the other hand, via secreting neuroprotective growth factors, mesenchymal stem cells (MSCs) have shown a promising potential in repairing central nervous system deficits. This study investigated the therapeutic effect of the combined use of MSCs and PRP in a rat model of SCI. We used real time-PCR method for evaluation of Bcl-2, Bax and caspase 3 expressions, TUNEL test for apoptotic cell death assessment, and neurofilament NF200 immunohistochemistry for examination of axonal regeneration. The results showed that co-treatment with MSCs and PRP efficiently alleviated the evaluated categories. Significant differences were observed in expression of Bcl-2 and caspase3, but not Bax, apoptotic index and the number of NF200 positive axons (for all P ≤ 0.01) between co-treatment animals compared with those treated with only MSCs or PRP. In conclusion, this study showed that combination of MSCs and PRP synergistically promotes their therapeutic effects in the SCI.

中文翻译：

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联合使用富含血小板的血浆和脂肪组织来源的间充质干细胞在实验性脊髓损伤中显示出协同作用

脊髓损伤 (SCI) 作为一种严重的残疾，会通过神经元丢失和纤维破坏导致组织退化。一些研究人员试图扭转或最小化这些变化。富血小板血浆（PRP）是一种来源于外周血的生物制品，含有多种生长因子。PRP已广泛用于再生医学。另一方面，通过分泌神经保护性生长因子，间充质干细胞 (MSCs) 在修复中枢神经系统缺陷方面显示出巨大潜力。本研究调查了 MSCs 和 PRP 联合使用在 SCI 大鼠模型中的治疗效果。我们使用实时 PCR 方法评估 Bcl-2、Bax 和 caspase 3 表达，使用 TUNEL 测试评估凋亡细胞死亡，并使用神经丝 NF200 免疫组织化学检查轴突再生。结果表明，与 MSCs 和 PRP 的共同处理有效地减轻了评估的类别。与仅用 MSCs 或 PRP 处理的动物相比，在 Bcl-2 和 caspase3 的表达上观察到显着差异，但在 Bax、凋亡指数和 NF200 阳性轴突的数量（对于所有 P ≤ 0.01）的数量上观察到显着差异。总之，这项研究表明，MSCs 和 PRP 的组合协同促进了它们在 SCI 中的治疗效果。