Abstract Clothianidin is a neonicotinoid insecticide that acts as a full agonist stimulating nAChRs and is widely used to control the brown planthopper, Nilaparvata lugens (Stal). To investigate the adaptation of N. lugens in the process of developing resistance to clothianidin, a clothianidin-resistant N. lugens strain and an unselected strain were used in this study. The F1 reciprocal crosses between the resistant and the susceptible strains indicated that the observed resistance was autosomal and incompletely dominant. Chi-square analyses of self-bred and backcrossed progenies suggested that the observed resistance was controlled by multiple genes. The resistant strain exhibited a relative fitness of 0.78 with a significantly increased adult preoviposition period and significantly decreased total fecundity. The current study provides valuable information for resistance management strategies to delay the development of clothianidin resistance in N. lugens.

中文翻译：

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褐飞虱 Nilaparvata lugens (Stål) 噻虫胺抗性的遗传模式和适应成本

摘要 Clothianidin 是一种新烟碱类杀虫剂，可作为刺激 nAChRs 的全激动剂，广泛用于防治褐飞虱 Nilaparvata lugens (Stal)。为了研究褐飞虱在对噻虫胺产生抗性过程中的适应性，在本研究中使用了耐噻虫胺的褐飞虱菌株和未选择的菌株。耐药菌株和易感菌株之间的 F1 互惠杂交表明观察到的耐药性是常染色体和不完全显性的。自交后代和回交后代的卡方分析表明观察到的抗性受多个基因控制。抗性菌株表现出 0.78 的相对适应性，成虫产卵前期显着增加，总繁殖力显着降低。